Caffeic acid phenethyl ester (CAPE) supplemented St. Thomas' hospital cardioplegic solution improves the antioxidant defense system of rat myocardium during ischemia-reperfusion injury.

Abstract

BACKGROUND AND AIM OF STUDY:

Cardioplegic arrest remains the method of choice for myocardial protection in cardiac surgery. Caffeic acid phenethyl ester (CAPE) prevents lipid peroxidation induced by ischemia-reperfusion injury and has a potent antioxidant property. We investigated the advantages of CAPE supplemented cardioplegic solution (St. Thomas' Hospital cardioplegic solution No.: 2) on the antioxidant defense system of myocardium against ischemia-reperfusion injury.

MATERIAL AND METHODS:

Isolated rat hearts were mounted on a nonrecirculating type of Langendorff apparatus. The hearts were arrested for 60 min with cardioplegic solution given at 20-min intervals and then reperfused for 15 min. The hearts were divided into three groups. Cold saline (0.9%, 4 degrees C) in group 1, St. Thomas' Hospital solution in group 2 and CAPE added St. Thomas' Hospital solution in group 3 were used as the cardioplegic solution. Krebs-Henseleit buffer solution was used for reperfusion. The tissues were examined biochemically for oxidative stress.

RESULTS:

Significant differences among the three groups existed in tissue myeloperoxidase (MPO), catalase (CAT), Na+-K+ ATPase activity and in the concentrations of malonydealdehyde (MDA) and 3-nitrotyrosine (3-NT). Group 2 showed significant changes in MPO (P = 0.04), Na+-K+ ATPase enzyme activity (P = 0.02) and the levels of MDA (P = 0.004) and 3-NT (P = 0.01) in comparison with group 1. Group 3 efficiently reduced MDA levels (P = 0.004) and also led to significant decrease in levels of MPO (P = 0.006), 3-NT (P = 0.01) and Na+-K+ ATPase activity (P = 0.01) and increase in the level of CAT (P = 0.004) in comparison with group 1. Significant changes were also found in the levels of MDA (P = 0.03), MPO (P = 0.04) and CAT (P = 0.009) in comparison between groups 2 and 3.

CONCLUSIONS:

We demonstrated that the administration of CAPE into cardioplegic solutions improves the antioxidant defense system of rat heart during the ischemia-reperfusion injury.

• Authors: Murat Ozeren, Nehir Sucu, Lülüfer Tamer, Barlas Aytacoglu, Ozgür Bayri, Ali Döndaş, Lokman Ayaz, Murat Dikmengil
• Journal: Pharmacological research (Pharmacol Res) Vol. 52 Issue 3 Pg. 258-63 (Sep 2005) ISSN: 1043-6618 [Print] Netherlands
• PMID: 15890527 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Caffeic Acids
• Cardioplegic Solutions
• Free Radical Scavengers
• 3-nitrotyrosine
• Tyrosine
• Malondialdehyde
• Catalase
• Peroxidase
• Sodium-Potassium-Exchanging ATPase
• caffeic acid phenethyl ester
• Phenylethyl Alcohol

Topics

• Animals
• Antioxidants (pharmacology)
• Caffeic Acids (pharmacology)
• Cardioplegic Solutions (pharmacology)
• Catalase (metabolism)
• Free Radical Scavengers (pharmacology)
• Heart (drug effects, physiology)
• In Vitro Techniques
• Male
• Malondialdehyde (metabolism)
• Myocardial Reperfusion Injury (metabolism, prevention & control)
• Peroxidase (metabolism)
• Phenylethyl Alcohol (analogs & derivatives, pharmacology)
• Rats
• Rats, Wistar
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Tyrosine (analogs & derivatives, metabolism)