Serum IgG mediates mucosal immunity against rotavirus infection.

Abstract

We evaluated the protective role of passively transferred circulating antibodies in protecting non-human primates against experimental rotavirus infection. Pooled sera with rotavirus-specific IgG titers that were either high (1:10,000), intermediate (1:300), or negative (< 1:25) were infused i.v. into naive pigtailed macaques (ages 3-6 months). Rotavirus-specific IgG could be detected in the sera at 18 h in all animals infused with antibody-containing serum, and fecal IgG titers could be detected only in animals given high-titer pooled sera. When orally challenged with 10(6) fluorescent-forming units of a simian rotavirus strain, YK-1, at 18 h after serum transfer, control animals shed virus starting 1-3 days after challenge and continued to shed virus at high titers for 6-8 days, whereas passively immunized macaques did not shed virus or had delayed shedding at low titers for only a limited time. The observation that passively transferred antibodies can suppress or delay viral infection in rotavirus-challenged pigtailed macaques has important implications for the design and testing of parenteral candidate rotavirus vaccines.

• Authors: Larry E Westerman, Harold M McClure, Baoming Jiang, Jeffrey W Almond, Roger I Glass
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 102 Issue 20 Pg. 7268-73 (May 17 2005) ISSN: 0027-8424 [Print] United States
• PMID: 15883382 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Immunoglobulin G

Topics

• Animals
• Antibodies, Viral (immunology)
• Feces (chemistry)
• Immunity, Mucosal (immunology)
• Immunoglobulin G (blood, immunology)
• Macaca nemestrina
• Neutralization Tests
• Rotavirus (immunology)
• Rotavirus Infections (immunology)
• Vaccination
• Virus Shedding (immunology)