Abstract | BACKGROUND: MATERIALS AND METHODS: It was examined if ectopic expression of human VEGF-D affected growth of breast carcinoma cell lines in vitro and in vivo. RESULTS:
VEGF-D overexpressing clonal MCF-7 and MDA-MB-231 cell lines displayed increased proliferative activities and upregulation of cyclins A, D1 and D3, compared to the vector control. Following subcutaneous inoculation of the MDA-MB-231 cells into nude mice, growth of the VEGF-D overexpressing cells was greatly accelerated. The tumor weight gain was accompanied by increased proliferative activity, cyclin A expression and microvascular density. CONCLUSIONS:
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Authors | Manabu Akahane, Takemi Akahane, Amy Shah, Eijiro Okajima, Unnur P Thorgeirsson |
Journal | Anticancer research
(Anticancer Res)
2005 Mar-Apr
Vol. 25
Issue 2A
Pg. 701-7
ISSN: 0250-7005 [Print] Greece |
PMID | 15868899
(Publication Type: Journal Article)
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Chemical References |
- CCND3 protein, human
- Ccnd3 protein, mouse
- Cyclin A
- Cyclin D3
- Cyclins
- DNA, Complementary
- Vascular Endothelial Growth Factor D
- Cyclin D1
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Topics |
- Animals
- Blotting, Western
- Breast Neoplasms
(blood supply, genetics, metabolism, pathology)
- Carcinoma
(blood supply, genetics, metabolism, pathology)
- Cell Growth Processes
(physiology)
- Cell Line, Tumor
- Cyclin A
(biosynthesis, genetics)
- Cyclin D1
(biosynthesis, genetics)
- Cyclin D3
- Cyclins
(biosynthesis, genetics)
- DNA, Complementary
(genetics)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Mice
- Mice, Nude
- Neovascularization, Pathologic
(metabolism, pathology)
- Transfection
- Up-Regulation
- Vascular Endothelial Growth Factor D
(biosynthesis, genetics, physiology)
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