Abstract | PURPOSE: EXPERIMENTAL DESIGN:
IGF-I and IGF-IR expression in gastrinomas from 54 patients with Zollinger-Ellison syndrome were analyzed and correlated with clinical/ tumor characteristics. IGF-I and IGF-IR mRNA levels were determined by competitive reverse transcription-PCR. IGF-IR expression, assessed by immunohistochemistry, was done on a subset. RESULTS: IGF-IR mRNA was found in 100% and IGF-I in 89%. IGF-I mRNA expression varied by >254-fold, IGF-IR by 2,670-fold, and the levels correlated in a given tumor. The IGF-IR level was lower in gastrinomas of patients who were rendered disease free and increased levels correlated with tumor growth, aggressiveness, extent, and with liver metastases. Increased IGF-I levels correlated with increased growth, tumor extent, and aggressiveness. Neither IGF-IR nor IGF-I levels correlated with tumor location, size, or its clinical/functional features. The IGF-IR correlated with disease-free survival. IGF-IRbeta was found in 31 of 32 tumors (97%) by immunohistochemistry. CONCLUSIONS: These results indicate that IGF-I and IGF-IR are expressed in almost all gastrinomas. Furthermore, assessment of IGF-I/IGF-IR expression in gastrinomas may be clinically useful in identifying those patients with more aggressive tumors who might benefit from more aggressive treatment.
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Authors | Masayuki Furukawa, Mark Raffeld, Carmen Mateo, Akio Sakamoto, Terry W Moody, Tetsuhide Ito, David J Venzon, Jose Serrano, Robert T Jensen |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 9
Pg. 3233-42
(May 01 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 15867218
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- RNA, Messenger
- Insulin-Like Growth Factor I
- Receptor, IGF Type 1
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Topics |
- Adolescent
- Adult
- Aged
- Cell Proliferation
- Disease-Free Survival
- Female
- Gastrinoma
(genetics, metabolism, pathology)
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Insulin-Like Growth Factor I
(genetics, metabolism)
- Male
- Middle Aged
- Neoplasm Metastasis
- Pancreatic Neoplasms
(genetics, metabolism, pathology)
- RNA, Messenger
(genetics, metabolism)
- Receptor, IGF Type 1
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
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