HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Calpain inhibition and insulin action in cultured human muscle cells.

Abstract
Variation in the calpain 10 gene has been reported to increase susceptibility to type 2 diabetes. Part of this susceptibility appears to be mediated by a decrease in whole body insulin sensitivity. As skeletal muscle is the primary tissue site of the peripheral insulin resistance in type 2 diabetes, the aim of this study was to use a human skeletal muscle cell culture system to explore the effects of calpain inhibition on insulin action. Calpain 10 mRNA and protein expression was examined in cultured myoblasts, myotubes, and whole skeletal muscle from non-diabetic subjects using RT-PCR and Western blotting. Changes in insulin-stimulated glucose uptake and glycogen synthesis in response to the calpain inhibitors ALLN and ALLM were measured. Calpain 10 expression was confirmed in cultured human myoblasts, myotubes, and native skeletal muscle. Insulin-stimulated glucose uptake was significantly decreased following preincubation with ALLN [404+/-40 vs 505+/-55 (mean+/-SEM)pmol/mg/min; with vs without ALLN: p = 0.04] and ALLM [455+/-38 vs 550+/-50 pmol/mg/min; with vs without ALLM: p = 0.025] in day 7 fused myotubes, but not in myoblasts. Neither ALLN nor ALLM affected insulin-stimulated glycogen synthesis in myoblasts or myotubes. These studies confirm calpain 10 expression in cultured human muscle cells and support a role for calpains in insulin-stimulated glucose uptake in human skeletal muscle cells that may be relevant to the pathogenesis of the peripheral insulin resistance in type 2 diabetes.
AuthorsL J Logie, A E Brown, S J Yeaman, M Walker
JournalMolecular genetics and metabolism (Mol Genet Metab) Vol. 85 Issue 1 Pg. 54-60 (May 2005) ISSN: 1096-7192 [Print] United States
PMID15862281 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA Primers
  • Insulin
  • RNA, Messenger
  • Glycogen
  • Calpain
  • calpain 10
  • Glucose
Topics
  • Base Sequence
  • Biological Transport
  • Calpain (antagonists & inhibitors, genetics)
  • Cell Culture Techniques
  • Cells, Cultured
  • DNA Primers
  • Diabetes Mellitus, Type 2 (genetics)
  • Gene Expression Regulation, Enzymologic
  • Genetic Predisposition to Disease
  • Glucose (metabolism)
  • Glycogen (biosynthesis)
  • Humans
  • Insulin (pharmacology)
  • Insulin Resistance (genetics)
  • Muscle, Skeletal (drug effects, physiology)
  • RNA, Messenger (genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: