Glycyrrhizin inhibits R5 HIV replication in peripheral blood monocytes treated with 1-methyladenosine.

Abstract

R5 HIV replicated in freshly isolated human peripheral blood monocytes after treatment with 1-methyladenosine (fresh PBM/MA), an immunosuppressive compound isolated from tumorous ascites fluids, while viral replication was not demonstrated in untreated peripheral blood monocytes (fresh PBM). The R5 HIV replication in fresh PBM/MA was inhibited by glycyrrhizin (GL). Without any other stimulation, fresh PBM/MA produced CCL2 and IL-10, while these soluble factors were not released from fresh PBM. GL greatly inhibited the production of CCL2 and IL-10 in fresh PBM/MA. After treatment with CCL2 and/or IL-10, CCR5 mRNA expression in fresh PBM was markedly enhanced, while only a trace level of the mRNA expression was detected in these cells in the absence of CCL2 or IL-10. CCR5 mRNA expression in fresh PBM treated with CCL2 or IL-10 was clearly inhibited by GL. These results indicate that GL inhibits R5 HIV replication in fresh PBM/MA through the inhibiting CCR5 expression mediated by CCL2 or IL-10.

• Authors: Miwa Takei, Makiko Kobayashi, Xiao-Dong Li, Richard B Pollard, Fujio Suzuki
• Journal: Pathobiology : journal of immunopathology, molecular and cellular biology (Pathobiology) Vol. 72 Issue 3 Pg. 117-23 ( 2005) ISSN: 1015-2008 [Print] Switzerland
• PMID: 15860928 (Publication Type: Journal Article)
• Copyright: Copyright (c) 2005 S. Karger AG, Basel.

Chemical References

• Anti-Infective Agents
• Chemokine CCL2
• RNA, Messenger
• Receptors, CCR5
• Interleukin-10
• 1-methyladenosine
• Glycyrrhizic Acid
• Adenosine

Topics

• Adenosine (analogs & derivatives, pharmacology)
• Anti-Infective Agents (pharmacology)
• Chemokine CCL2 (biosynthesis, pharmacology)
• Gene Expression (drug effects)
• Glycyrrhizic Acid (pharmacology)
• HIV (drug effects, growth & development)
• Humans
• Interleukin-10 (biosynthesis, pharmacology)
• Leukocytes, Mononuclear (drug effects, metabolism, virology)
• RNA, Messenger (genetics, metabolism)
• Receptors, CCR5 (genetics)
• Virus Replication (drug effects)