Effect of IGL-1, a new preservation solution, on kidney grafts (a pre-clinical study).

Abstract

Ischemia-reperfusion injury conditions short-term and long-term graft function. The effects of the inversion of K+ and Na+ concentrations and substitution with polyethylene glycol for hydroxyethyl starch in University of Wisconsin (K-UW) solution were evaluated in isolated perfused rat kidneys and in autotransplanted pig kidneys. In the rat model kidneys were cold-stored for 24 h in K-UW or Na-UW or Na-PEG UW solutions (IGL-1 solution). Fractional sodium reabsorption and glomerular filtration rate was better in kidneys preserved in Na-UW and IGL-1 solution than those preserved in K-UW solution. In the pig model the left kidney was harvested and preserved in K-UW or IGL-1 solution for 24 h and then transplanted. In the autotransplanted pig model, kidneys preserved in IGL-1 solution showed a better function and a significant reduction of MHC class II expression, cellular apoptosis and interstitial fibrosis. In conclusion, kidneys preserved in IGL-1 solution tolerated ischemia/reperfusion injury better than those preserved in K-UW solution.

• Authors: Lionel Badet, Hassen Ben Abdennebi, Palmina Petruzzo, Brigitte McGregor, Michele Espa, Aoumeur Hadj-Aissa, Silvina Ramella-Virieux, Jean Paul Steghens, Francesca Portoghese, Xavier Martin
• Journal: Transplant international : official journal of the European Society for Organ Transplantation (Transpl Int) Vol. 17 Issue 12 Pg. 815-21 (May 2005) ISSN: 0934-0874 [Print] Switzerland
• PMID: 15856175 (Publication Type: Journal Article)

Chemical References

• IGL-1 solution
• Organ Preservation Solutions

Topics

• Animals
• Drug Evaluation, Preclinical
• In Vitro Techniques
• Kidney Transplantation
• Male
• Models, Animal
• Organ Preservation Solutions (chemistry, pharmacology)
• Perfusion
• Rats
• Rats, Sprague-Dawley
• Swine
• Transplantation, Autologous