Differential labeling by monoclonal antibodies Adnab-9 and anti-alpha-defensin 5 based on the distribution and adenomatous tissue content of colonic polyps.

Abstract

We sought a correlation between site and morphology of colonic polyps by labeling with neoplastic and general Paneth cell markers, monoclonal antibodies Adnab-9 and anti-alpha-defensin 5, respectively. Proportions labeled by Adnab-9 and anti-alpha-defensin 5 were, respectively, 42 and 85% for adenomas, 39 and 63% for early tubular adenomas, 41 and 44% for serrated, 34 and 20% for mixed, and 11 versus 2.7% for hyperplastic polyps. Compared with hyperplastic polyps, the proportion of other polyps labeled by Adnab-9 or anti-alpha-defensin 5 was higher but this difference was more significant for distal (P = 0.008 for Adnab-9 and P = 0.0001 for anti-alpha-defensin 5) than proximal (P = 0.645 and P = 0.154, respectively) polyps. While increased labeling of all proximal polyps compared to distal ones mirrored the colonic distribution of Paneth cells, distal adenomas tended to have a higher proportion labeled by Adnab-9, suggesting that Adnab-9 labels Paneth cells associated with increased neoplastic potential.

• Authors: Nadeem Ullah, Kashif Qureshi, Violeta Yordanova, James Hatfield, Paula Sochacki, Michael Lawson, Martin Tobi
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 50 Issue 4 Pg. 708-13 (Apr 2005) ISSN: 0163-2116 [Print] United States
• PMID: 15844706 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Monoclonal
• DEFA5 protein, human
• alpha-Defensins

Topics

• Adenomatous Polyps (pathology)
• Antibodies, Monoclonal
• Colonic Neoplasms (pathology)
• Colonic Polyps (pathology)
• Diagnosis, Differential
• Humans
• Immunohistochemistry (methods)
• Paneth Cells (pathology)
• Single-Blind Method
• Staining and Labeling
• alpha-Defensins (immunology)