Risk factors for Buruli ulcer disease (Mycobacterium ulcerans Infection): results from a case-control study in Ghana.

Abstract

BACKGROUND:

Morbidity due to Buruli ulcer disease (BUD), a cutaneous infection caused by Mycobacterium ulcerans, has been increasingly recognized in rural West Africa. The source and mode of transmission remain unknown.

METHODS:

To identify BUD risk factors, we conducted a case-control study in 3 BUD-endemic districts in Ghana. We enrolled case patients with clinically diagnosed BUD and obtained skin biopsy specimens. M. ulcerans infection was confirmed by at least 1 of the following diagnostic methods: histopathologic analysis, culture, polymerase chain reaction, and Ziehl-Neelsen staining of a lesion smear. We compared characteristics of case patients with confirmed BUD with those of age- and community-matched control subjects using conditional logistic regression analysis.

RESULTS:

Among 121 case patients with confirmed BUD, leg lesions (49%) or arm lesions (36%) were common. Male case patients were significantly more likely than female case patients to have lesions on the trunk (25% vs. 6%; P = .009). Multivariable modeling among 116 matched case-control pairs identified wading in a river as a risk factor for BUD (odds ratio [OR], 2.69; 95% confidence interval [CI], 1.27-5.68; P = .0096). Wearing a shirt while farming (OR, 0.27; 95% CI, 0.11-0.70; P = .0071), sharing indoor living space with livestock (OR, 0.36; 95% CI, 0.15-0.86; P = .022), and bathing with toilet soap (OR, 0.41; 95% CI, 0.19-0.90; P = .026) appeared to be protective. BUD was not significantly associated with penetrating injuries (P = .14), insect bites near water bodies (P = .84), bacille Calmette-Guerin vaccination (P = .33), or human immunodeficiency virus infection (P = .99).

CONCLUSIONS:

BUD is an environmentally acquired infection strongly associated with exposure to river areas. Exposed skin may facilitate transmission. Until transmission is better defined, control strategies in BUD-endemic areas could include covering exposed skin.

• Authors: Pratima L Raghunathan, Ellen A S Whitney, Kwame Asamoa, Ymkje Stienstra, Thomas H Taylor Jr, George K Amofah, David Ofori-Adjei, Karen Dobos, Jeannette Guarner, Stacey Martin, Sonal Pathak, Erasmus Klutse, Samuel Etuaful, Winette T A van der Graaf, Tjip S van der Werf, C H King, Jordan W Tappero, David A Ashford
• Journal: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (Clin Infect Dis) Vol. 40 Issue 10 Pg. 1445-53 (May 15 2005) ISSN: 1537-6591 [Electronic] United States
• PMID: 15844067 (Publication Type: Journal Article)

Topics

• Adolescent
• Adult
• Case-Control Studies
• Child
• Child, Preschool
• Female
• Ghana (epidemiology)
• Humans
• Infant
• Male
• Middle Aged
• Multivariate Analysis
• Mycobacterium Infections, Nontuberculous (epidemiology, microbiology)
• Mycobacterium ulcerans (isolation & purification)
• Odds Ratio
• Risk Factors
• Sex Characteristics
• Skin Ulcer (epidemiology, microbiology)