Abstract |
Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r- flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r- flagellin, or only to r- flagellin, whereas patients with early disease showed no response or responded only to r- flagellin. Patients who developed antibodies to r- flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.
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Authors | E Fikrig, E D Huguenel, R Berland, D W Rahn, J A Hardin, R A Flavell |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 165
Issue 6
Pg. 1127-32
(Jun 1992)
ISSN: 0022-1899 [Print] United States |
PMID | 1583333
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Bacterial
- Antibodies, Monoclonal
- Antigens, Bacterial
- Bacterial Outer Membrane Proteins
- Recombinant Fusion Proteins
- Recombinant Proteins
- Flagellin
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Topics |
- Antibodies, Bacterial
(blood)
- Antibodies, Monoclonal
(immunology)
- Antigens, Bacterial
(immunology)
- Bacterial Outer Membrane Proteins
(immunology)
- Borrelia burgdorferi Group
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Flagellin
(immunology)
- Humans
- Immunoblotting
- Lyme Disease
(diagnosis)
- Recombinant Fusion Proteins
(immunology)
- Recombinant Proteins
(immunology)
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