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Serologic diagnosis of Lyme disease using recombinant outer surface proteins A and B and flagellin.

Abstract
Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.
AuthorsE Fikrig, E D Huguenel, R Berland, D W Rahn, J A Hardin, R A Flavell
JournalThe Journal of infectious diseases (J Infect Dis) Vol. 165 Issue 6 Pg. 1127-32 (Jun 1992) ISSN: 0022-1899 [Print] United States
PMID1583333 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Flagellin
Topics
  • Antibodies, Bacterial (blood)
  • Antibodies, Monoclonal (immunology)
  • Antigens, Bacterial (immunology)
  • Bacterial Outer Membrane Proteins (immunology)
  • Borrelia burgdorferi Group (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Flagellin (immunology)
  • Humans
  • Immunoblotting
  • Lyme Disease (diagnosis)
  • Recombinant Fusion Proteins (immunology)
  • Recombinant Proteins (immunology)

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