Abstract | BACKGROUND: MATERIALS AND METHODS: To further characterize this model, rats received two injections of indomethacin (7.5 mg kg(-1)) 24 h apart and histological damage of intestinal mucosa, oxidative stress, alterations of intestinal motility and mesenteric vascular bed (MVB) reactivity were investigated after 5 days. RESULTS: The results show that indomethacin caused several histological and functional changes at the ileal level. In particular, response to carbachol as well as the nonadrenergic-noncholinergic inhibitory response to electrical field stimulation (EFS) was lower in the treated than control rats. Moreover, nitric oxide (NO)-component of the inhibitory response was higher in the treated than control rats. Mesenteric vessels preparations from the treated rats showed increased noradrenaline (NA)-induced perfusion pressure, whereas relaxant responses to acetylcholine, although not significantly reduced in the treated rats, had a higher nitrergic component. This finding suggests that vascular dysfunction may contribute to chronic inflammation. Indomethacin injection also determined acute and severe oxidative stress in ileum mucosa. CONCLUSIONS: In conclusion, our study contributes to further characterize the rat model of indomethacin-induced enteritis and suggests that it is suitable for drug screening in rats, as this model can be obtained in a very short period and is simple and reproducible.
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Authors | A L Piepoli, G De Salvatore, M A De Salvia, C I Mitolo, G Siro-Brigiani, A Marzullo, I Grattagliano, D Mitolo-Chieppa, G Palasciano, P Portincasa |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 35
Issue 4
Pg. 271-8
(Apr 2005)
ISSN: 0014-2972 [Print] England |
PMID | 15816997
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adrenergic alpha-Agonists
- Cholinergic Agonists
- Carbachol
- Acetylcholine
- Norepinephrine
- Indomethacin
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Topics |
- Acetylcholine
(metabolism)
- Adrenergic alpha-Agonists
(pharmacology)
- Animals
- Carbachol
(pharmacology)
- Cholinergic Agonists
(pharmacology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Gastrointestinal Motility
(drug effects, physiology)
- Ileum
(drug effects, pathology, physiopathology)
- Indomethacin
- Inflammatory Bowel Diseases
(pathology, physiopathology)
- Intestinal Mucosa
(drug effects, pathology)
- Mesentery
(blood supply, drug effects)
- Muscle Contraction
(drug effects)
- Muscle, Smooth
(physiopathology)
- Norepinephrine
(pharmacology)
- Oxidative Stress
(drug effects, physiology)
- Rats
- Rats, Wistar
- Synaptic Transmission
(drug effects, physiology)
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