Anti-alopecia mechanisms of soymetide-4, an immunostimulating peptide derived from soy beta-conglycinin.

Abstract

Previously, we found that orally administered soymetide-4 (MITL), an immunostimulating peptide derived from soybean beta-conglycinin alpha' subunit, suppressed alopecia induced by the anti-cancer drug etoposide in neonatal rats. Soymetide-4 has weak affinity for N-formyl-methionyl-leucyl-phenylalanine (fMLP) receptor. fMLP showed an anti-alopecia effect after intraperitoneal administration, though it was inactive after oral administration. Anti-alopecia effect of fMLP was blocked by pyrilamine or cimetidine, antagonists for histamine H1 or H2 receptor, respectively. However, the anti-alopecia effect of soymetide-4 was not inhibited by the histamine antagonists but by indomethacin, an inhibitor of cyclooxygenase (COX), or AH-23848B, an antagonist of the EP4 receptor for PGE2. Anti-alopecia effect of soymetide-4 was also blocked by pyrrolidine dithiocarbamate, an inhibitor of nuclear factor-kappaB (NF-kappaB). These results suggest that PGE2, which is produced after activation of COX by soymetide-4, might suppress apoptosis of hair matrix cells and etoposide-induced alopecia by activating NF-kappaB.

• Authors: Takahiro Tsuruki, Kyoya Takahata, Masaaki Yoshikawa
• Journal: Peptides (Peptides) Vol. 26 Issue 5 Pg. 707-11 (May 2005) ISSN: 0196-9781 [Print] United States
• PMID: 15808899 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antigens, Plant
• Antineoplastic Agents, Phytogenic
• Biphenyl Compounds
• Cyclooxygenase Inhibitors
• Globulins
• Histamine H1 Antagonists
• NF-kappa B
• Peptide Fragments
• Pyrrolidines
• Receptors, Formyl Peptide
• Receptors, Prostaglandin E
• Receptors, Prostaglandin E, EP4 Subtype
• Seed Storage Proteins
• Soybean Proteins
• Thiocarbamates
• beta-conglycinin protein, Glycine max
• soymetide-4
• pyrrolidine dithiocarbamic acid
• N-Formylmethionine Leucyl-Phenylalanine
• Etoposide
• AH 23848
• Prostaglandin-Endoperoxide Synthases
• Indomethacin

Topics

• Adjuvants, Immunologic (pharmacology, therapeutic use)
• Administration, Oral
• Alopecia (prevention & control)
• Animals
• Antigens, Plant
• Antineoplastic Agents, Phytogenic (toxicity)
• Biphenyl Compounds (pharmacology)
• Cyclooxygenase Inhibitors (pharmacology)
• Etoposide (toxicity)
• Female
• Globulins (chemistry)
• Histamine H1 Antagonists (pharmacology)
• Indomethacin (pharmacology)
• Male
• N-Formylmethionine Leucyl-Phenylalanine (pharmacology)
• NF-kappa B (antagonists & inhibitors)
• Peptide Fragments (administration & dosage, pharmacology, therapeutic use)
• Prostaglandin-Endoperoxide Synthases (drug effects, metabolism)
• Pyrrolidines (pharmacology)
• Rats
• Receptors, Formyl Peptide (drug effects)
• Receptors, Prostaglandin E (antagonists & inhibitors, metabolism)
• Receptors, Prostaglandin E, EP4 Subtype
• Seed Storage Proteins
• Soybean Proteins (administration & dosage, chemistry, pharmacology, therapeutic use)
• Thiocarbamates (pharmacology)