Isolation of hepatocyte-like cells from mouse embryoid body cells.

Abstract

We previously reported that embryoid body (EB) cells derived from embryonic stem (ES) cells are capable of differentiating into functional hepatocyte-like cells both in vitro and in vivo. Because transplantation of EB-derived cells into the liver via the spleen resulted in a low incidence of teratoma formation, purification of hepatocyte-like cells is required to prevent teratoma formation. The aim of this study was to purify hepatocyte-like cells from cultured EBs. For the isolation of hepatocyte-like cells, EBs cultured for 15 days were treated with trypsin-EDTA. The disaggregated cells were plated on a gelatin-coated dish as a monolayer. These cells were separated by Percoll gradient centrifugation, enriched by magnetic cell sorting, and purified by FACS. The purified hepatocyte-like cells in monolayer cultures were positive for immunostaining for albumin and expressed albumin mRNA, but not Oct3/4 mRNA. Transplantation of the purified hepatocyte-like cells derived from mouse ES cells might be an effective treatment for liver failure.

• Authors: Y Kumashiro, K Teramoto, K Shimizu-Saito, K Asahina, H Teraoka, S Arii
• Journal: Transplantation proceedings (Transplant Proc) 2005 Jan-Feb Vol. 37 Issue 1 Pg. 299-300 ISSN: 0041-1345 [Print] United States
• PMID: 15808625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers

Topics

• Animals
• Base Sequence
• Cell Differentiation
• Cell Line
• Cell Separation (methods)
• DNA Primers
• Flow Cytometry
• Hepatocytes (cytology)
• Liver (embryology)
• Mice
• Stem Cells (cytology)