Anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia/reperfusion injury in rats.

Abstract

The objective of this study is to investigate the anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia injury in rats and to explore its mechanisms of action. After 24 h of reperfusion following 2 h of cerebral ischemia, the infiltration of neutrophils was observed by myeloperoxidase (MPO) activity determination, the expression of intercellular adhesion molecule-1 (ICAM-1) was observed by western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, and the nuclear translocation and DNA binding activity of nuclear factor-kappaB (NF-kappaB) were observed by western blot and electrophoretic mobility shift assay (EMSA). The results showed that hydroxyethylpuerarin could obviously inhibit the MPO activity and ICAM-1 expression following 2 hours of ischemia with 24 hours of reperfusion. The nuclear translocation and DNA binding activity were also decreased by hydroxyethylpuerarin treatment. These results suggested that hydroxyethylpuerarin could inhibit neutrophil-mediated inflammatory response after brain ischemia reperfusion in rats. This effect may be mediated by down-regulation of ICAM-1 and NF-kappaB activity.

• Authors: Hai-Yan Lou, Xiu-Mei Zhang, Xin-Bing Wei, Ru-Xia Wang, Xia Sun
• Journal: The Chinese journal of physiology (Chin J Physiol) Vol. 47 Issue 4 Pg. 197-201 (Dec 31 2004) ISSN: 0304-4920 [Print] India
• PMID: 15803753 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Isoflavones
• NF-kappa B
• Neuroprotective Agents
• Oncogene Protein p65(gag-jun)
• Intercellular Adhesion Molecule-1
• DNA
• Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Blotting, Western
• Brain (enzymology, pathology)
• Brain Chemistry (drug effects)
• Brain Ischemia (pathology)
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• DNA (metabolism)
• Electrophoretic Mobility Shift Assay
• Intercellular Adhesion Molecule-1 (biosynthesis)
• Isoflavones (pharmacology)
• Male
• NF-kappa B (biosynthesis)
• Neuroprotective Agents
• Oncogene Protein p65(gag-jun) (biosynthesis)
• Peroxidase (metabolism)
• Rats
• Reperfusion Injury (pathology)
• Reverse Transcriptase Polymerase Chain Reaction