Abstract | BACKGROUND:
Heme oxygenase-1 (HO-1) seems to have an important protective role in acute and chronic inflammation. The products of heme catalysis, biliverdin/ bilirubin, carbon monoxide (CO), and iron (that induces apoferritin) mediate the beneficial effects of HO-1. Blockade of HO-1 activity results in exacerbation of experimental colitis. We tested whether HO-1 has protective effects in the development of colitis and determined that specific enzymatic products of HO-1 are responsible for these effects. METHODS: RESULTS:
Cobalt-protoporphyrin treatment resulted in significant up-regulation of HO-1 protein in mucosal and submucosal cells. Induction of HO-1 was associated with significantly less loss of body weight in mice with induced colitis (-12% versus -22% in the control animals, P < 0.001). Development of diarrhea and gastrointestinal hemorrhage was substantially delayed in animals in which HO-1 was induced, and mucosal injury was significantly attenuated. Administration of CO or desferrioxamine alone had no significant effects, whereas enhanced protection with lesser evidence of bowel inflammation was observed with systemic biliverdin administration (50 micromol/kg, 3 times per day, intraperitoneally). CONCLUSIONS:
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Authors | Pascal O Berberat, Yousif I A-Rahim, Kenichiro Yamashita, Michel M Warny, Eva Csizmadia, Simon C Robson, Fritz H Bach |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 11
Issue 4
Pg. 350-9
(Apr 2005)
ISSN: 1078-0998 [Print] England |
PMID | 15803024
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Membrane Proteins
- Protoporphyrins
- zinc protoporphyrin
- cobaltiprotoporphyrin
- Heme Oxygenase (Decyclizing)
- Heme Oxygenase-1
- Hmox1 protein, mouse
- Biliverdine
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Topics |
- Acute Disease
- Animals
- Biliverdine
(physiology)
- Colitis
(enzymology, pathology, prevention & control)
- Disease Models, Animal
- Heme Oxygenase (Decyclizing)
(antagonists & inhibitors, drug effects, physiology)
- Heme Oxygenase-1
- Intestinal Mucosa
(enzymology, pathology)
- Male
- Membrane Proteins
- Mice
- Mice, Inbred C57BL
- Protoporphyrins
(pharmacology)
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