Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3.

Abstract

In order to improve the sensitivity and stability of human blood samples containing WR-1065 (i.e., active metabolite of the cytoprotective agent amifostine), a high-performance liquid chromatographic method was developed and validated using fluorescent derivatization with ThioGlo3. Using a sample volume of only 100 microl, the method was specific, sensitive (limit of quantitation=10 nM in deproteinized blood or 20 nM in whole blood), accurate (error < or = 3.2%) and reproducible (CV < or = 8.7%). In addition, the stability of WR-1065 in deproteinized and derivatized blood samples was assured for at least four weeks at -20 degrees C. This method should be particularly valuable in translating the kinetic-dynamic relationship of WR-1065 in preclinical models to that in cancer patients.

• Authors: Jun Chen, Zheng Lu, Theodore S Lawrence, David E Smith
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 819 Issue 1 Pg. 161-7 (May 05 2005) ISSN: 1570-0232 [Print] Netherlands
• PMID: 15797534 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S., Validation Study)

Chemical References

• 9-acetoxy-2-(4-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)phenyl)-3-oxo-3H-naphtho(2,1-b)pyran
• Mercaptoethylamines
• Pyrans
• Pyrroles
• N-(2-mercaptoethyl)-1,3-diaminopropane
• Amifostine

Topics

• Amifostine (metabolism, therapeutic use)
• Chromatography, High Pressure Liquid (instrumentation, methods)
• Drug Stability
• Humans
• Liver Neoplasms (drug therapy)
• Mercaptoethylamines (blood)
• Pyrans
• Pyrroles
• Reproducibility of Results
• Sensitivity and Specificity
• Spectrometry, Fluorescence