Abstract | OBJECTIVE: METHODS: Using immunohistochemistry, expression of DDH was measured in 41 patients with epithelial ovarian cancers. All patients underwent primary debulking surgery, followed with six cycles of cisplatin-based chemotherapy. Normal ovarian tissues were obtained from patients with benign gynecologic diseases (n = 14). Expression of DDH was confirmed by reverse transcription-polymerase chain reaction. The correlation between DDH expression and clinico-pathological parameters was analyzed by statistical analysis. Difference of progression-free survivals between different groups was compared by a log-rank test. RESULTS: Eighteen ovarian cancer samples (43.9%) expressed DDH at a moderate to strong level. This marked a significant difference from the negligible expression (1/14, 7.1%) found in the control group (P = 0.02). Of interest, the clear cell adenocarcinoma revealed DDH overexpression (75%) and mucinous adenocarcinoma revealed low DDH expression (16.7%), although DDH expression did not show any significant variation according to different histotypes. DDH overexpression was found in a statistically significantly higher percentage of cisplatin-resistant cases (n = 8/11; 72.7%) than in cisplatin-sensitive cases (n = 9/27; 33.3%) (P = 0.037). Using multivariate analysis, only DDH retained as an independent role in predicting a poor chance of response to cisplatin-based treatment. DDH overexpression cases (median 12 months, 95% confidence interval 4-20) demonstrated a shorter progression-free survival than DDH-negative cases (median 28 months, 95% confidence interval 23-33), but this result did not reach the statistical significance (P = 0.1742). In the advance stage, the DDH-positive group has a shorter PFS as compared with DDH-negative group, and this result closely approaches the statistical significance (P = 0.0669). CONCLUSIONS: DDH is expressed in a high percentage of primary ovarian tumors and its expression may be associated with cisplatin-based chemotherapy resistance. The possible prognostic role of DDH in ovarian carcinoma deserves further study.
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Authors | Yi-Jen Chen, Chiou-Chung Yuan, Kuan-Chih Chow, Peng-Hui Wang, Chiung-Ru Lai, Ming-Shyen Yen, Liang-Shun Wang |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 97
Issue 1
Pg. 110-7
(Apr 2005)
ISSN: 0090-8258 [Print] United States |
PMID | 15790446
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- RNA, Messenger
- Oxidoreductases
- trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
- Cisplatin
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Cisplatin
(administration & dosage, pharmacology)
- DNA, Neoplasm
(genetics)
- Drug Resistance, Neoplasm
- Female
- Humans
- Immunohistochemistry
- Middle Aged
- Neoplasm Staging
- Ovarian Neoplasms
(drug therapy, enzymology, genetics, pathology)
- Oxidoreductases
(biosynthesis, genetics)
- RNA, Messenger
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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