Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection.

Abstract

We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotarnine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotarnine were less than 4.8%. The method developed here was better than the method reported previously.

• Authors: Hideya Kokubun, Makiko Ouki, Motohiro Matoba, Hiroaki Kubo, Sumio Hoka, Kazuo Yago
• Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (Anal Sci) Vol. 21 Issue 3 Pg. 337-9 (Mar 2005) ISSN: 0910-6340 [Print] Switzerland
• PMID: 15790123 (Publication Type: Journal Article)

Chemical References

• Analgesics, Opioid
• Tetrahydroisoquinolines
• Oxycodone
• hydrocotarnine

Topics

• Analgesics, Opioid (blood)
• Chromatography, High Pressure Liquid (methods)
• Electrochemistry (methods)
• Humans
• Neoplasms (blood)
• Oxycodone (blood)
• Tetrahydroisoquinolines (blood)