| Abstract | The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format. |
| Authors | Edneia C Bueno, Christina M Scheel, Adelaide J Vaz, Luis R Machado, José A Livramento, Osvaldo M Takayanagui, Victor C W Tsang, Kathy Hancock
(Affiliation: Faculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil. ecbueno at ccs.univali.br)
|
| Journal | The American journal of tropical medicine and hygiene
(Am J Trop Med Hyg)
Vol. 72
Issue 3
Pg. 278-83
(Mar 2005)
ISSN: 0002-9637 United States |
| PMID | 15772321
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antibodies
- Nerve Tissue Proteins
- Recombinant Proteins
- brain synaptic membrane glycoprotein gp 50
|
| Topics |
- Animals
- Antibodies
(blood)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Humans
- Nerve Tissue Proteins
(blood, genetics)
- Neurocysticercosis
(blood, diagnosis, immunology)
- Recombinant Proteins
(blood)
- Reference Values
- Sensitivity and Specificity
- Serologic Tests
- Taenia
(immunology)
- Taeniasis
(blood, diagnosis, immunology)
|
