Abstract |
Kaurene-type diterpenes possess various biological activities including antitumor and anti-inflammatory effects. Indeed, we have found that an ent-kaurene diterpene, ent-11alpha-hydroxy-16-kauren-15-one (KD), induced apoptosis via caspase-8 activation in human promyelocytic leukemia HL-60 cells. However, the mechanism of caspase-8 activation by KD is not clear. In this study, we investigated the involvement of p38 mitogen-activated protein kinase ( p38 (MAPK)) in KD-induced apoptosis. p38 (MAPK) was activated by treatment with KD parallel to DNA ladder formation. Pretreatment with SB203580, a specific inhibitor of p38 (MAPK), attenuated induction of apoptosis by KD and inhibited activation of caspase-8. Cleavage of Bid, a typical substrate of caspase-8, was also inhibited by treatment with SB203580, suggesting that activation of p38 (MAPK) occurs upstream of caspase-8 during KD-induced apoptosis.
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Authors | Masuo Kondoh, Ikue Suzuki, Motoki Harada, Fumihiro Nagashima, Makiko Fujii, Yoshinori Asakawa, Yoshiteru Watanabe |
Journal | Planta medica
(Planta Med)
Vol. 71
Issue 3
Pg. 275-7
(Mar 2005)
ISSN: 0032-0943 [Print] Germany |
PMID | 15770551
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Diterpenes, Kaurane
- Mitogens
- Plant Extracts
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Antineoplastic Agents, Phytogenic
(administration & dosage, pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Diterpenes, Kaurane
(administration & dosage, pharmacology, therapeutic use)
- HL-60 Cells
(drug effects, metabolism)
- Humans
- Isodon
- Mitogens
(administration & dosage, pharmacology, therapeutic use)
- Phytotherapy
- Plant Extracts
(administration & dosage, pharmacology, therapeutic use)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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