TATA-binding protein (TBP)-like factor (TLF) is a functional regulator of transcription: reciprocal regulation of the neurofibromatosis type 1 and c-fos genes by TLF/TRF2 and TBP.

Abstract	The lack of direct targets for TATA-binding protein (TBP)-like factors (TLFs) confounds the understanding of their role in gene expression. Here we report that human TLF (also called TBP-related factor 2 [TRF2]) activates a number of different genes, including the neurofibromatosis type 1 (NF1) gene. The overexpression of TLF increases the amount of NF1 mRNA in cells. In vivo, TLF binds to and upregulates transcription from a fragment of the NF1 promoter. In vitro, purified TLF-TFIIA binds directly to the same NF1 promoter fragment that is required for TLF responsiveness in cells. Furthermore, targeted deletion of TLF in mice reduces NF1 levels. In contrast, TLF inhibits transcription driven by a fragment from the TATA-containing c-fos promoter by sequestering TFIIA. TBP affects the NF1 and c-fos promoters in a manner reciprocal to that of TLF, stimulating the c-fos promoter and inhibiting NF1 transcription. We conclude that TLF is a functional regulator of transcription with targets distinct from those of TBP.
Authors	Jayhong A Chong, Magdalene M Moran, Martin Teichmann, J Stefan Kaczmarek, Robert Roeder, David E Clapham
Journal	Molecular and cellular biology (Mol Cell Biol) Vol. 25 Issue 7 Pg. 2632-43 (Apr 2005) ISSN: 0270-7306 [Print] United States
PMID	15767669 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Proto-Oncogene Proteins c-fos TATA Box Binding Protein-Like Proteins TATA-Box Binding Protein TBPL1 protein, human
Topics	Amino Acid Sequence Animals Base Sequence Cell Line Chlorocebus aethiops Gene Expression Regulation (genetics) Genes, Neurofibromatosis 1 Humans Mice Molecular Sequence Data Promoter Regions, Genetic (genetics) Protein Binding Proto-Oncogene Proteins c-fos (genetics) TATA Box Binding Protein-Like Proteins (chemistry, genetics, metabolism) TATA-Box Binding Protein (metabolism) Transcription, Genetic (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!