Shiquandabutangjiaweibang inhibits tumor metastasis and angiogenesis via regulation of topoisomerase-1.

Abstract

Shiquandabutangjiaweibang (SDJ) is a traditional medicine prescription used for increasing body resistance against cancer. In the present study, the effect of SDJ extract on tumor metastasis and angiogenesis was evaluated. SDJ showed cytotoxicity against P388 (leukemia cells) and B16-F10 (murine melanoma cells) to 60% of control at 1 mg. SDJ significantly inhibited lung metastasis and also restored the number of platelets in C57BL/6 mice with thrombocytopenia induced by intravenous injection of B16-F10 cells. SDJ significantly disrupted chick embryonic angiogenesis in the chorioallantoic membrane (CAM). Interestingly, SDJ suppressed DNA topoisomerase I in a concentration-dependent manner. These results suggest that SDJ can be a potent inhibitor of metastasis and angiogenesis, at least in part, via regulation of topoisomerase I.

• Authors: Sung Hoon Kim, Tae-Hyung Lee, Deok-Chun Yang, Hyung-Min Kim, Jeung-Beum Kim, Mi-Kyung Park, Yong-Soo Bae
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 98 Issue 1-2 Pg. 157-62 (Apr 08 2005) ISSN: 0378-8741 [Print] Ireland
• PMID: 15763377 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Plant Preparations
• shiquandabutangjiaweibang
• DNA Topoisomerases, Type I

Topics

• Animals
• Cell Survival (drug effects)
• Chorioallantoic Membrane (drug effects)
• DNA Topoisomerases, Type I (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor (methods)
• Humans
• Leukemia P388 (pathology, prevention & control)
• Lung Neoplasms (prevention & control, secondary)
• Male
• Medicine, East Asian Traditional
• Mice
• Mice, Inbred C57BL
• Neoplasm Metastasis (prevention & control)
• Neoplasm Transplantation (methods)
• Neoplasms, Experimental (drug therapy)
• Neoplastic Cells, Circulating (pathology)
• Neovascularization, Pathologic (prevention & control)
• Plant Preparations (analysis, chemistry, pharmacology)
• Staining and Labeling
• Thrombocytopenia (chemically induced)
• Thrombocytosis (chemically induced)
• Tumor Cells, Cultured