Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum.

Abstract

Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae) causes human, equine and canine granulocytic anaplasmosis and tick-borne fever of ruminants. The rickettsia parasitizes granulocytes and bone marrow progenitor cells, and can be propagated in human promyelocytic and tick cell lines. In this study, microarrays of synthetic polynucleotides of 21,329 human genes were used to identify genes that are differentially expressed in HL-60 human promyelocytic cells in response to infection with A. phagocytophilum. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) of selected genes confirmed the results of the microarray analysis. Six genes in the A. phagocytophilum-infected cells were found to be upregulated greater than 30-fold, while expression of downregulated genes most often did not change more than sixfold. Genes that were found to be differentially regulated in infected cells were those essential for cellular mechanisms including growth and differentiation, cell transport, signalling and communication and protective response against infection, some of which are most likely necessary for infection and multiplication of A. phagocytophilum in host cells. The differentially regulated genes described herein provide new information on the gene expression profiles in A. phagocytophilum-infected HL-60 cells, thus expanding in a global manner the existing information on the response of mammalian cells to A. phagocytophilum infection.

• Authors: José de la Fuente, Patricia Ayoubi, Edmour F Blouin, Consuelo Almazán, Victoria Naranjo, Katherine M Kocan
• Journal: Cellular microbiology (Cell Microbiol) Vol. 7 Issue 4 Pg. 549-59 (Apr 2005) ISSN: 1462-5814 [Print] India
• PMID: 15760455 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Neoplasm Proteins

Topics

• Anaplasma phagocytophilum (pathogenicity)
• Gene Expression Profiling
• Gene Expression Regulation
• Granulocyte Precursor Cells (microbiology)
• HL-60 Cells (microbiology)
• Humans
• Neoplasm Proteins (genetics, metabolism)
• Oligonucleotide Array Sequence Analysis