Identifying relapsing fever Borrelia, Senegal.

Abstract

We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagnosed with spirochete infections. Borrelia crocidurae was the only species identified.

• Authors: Hamoud Brahim, Jean David Perrier-Gros-Claude, Danielle Postic, Guy Baranton, Ronan Jambou
• Journal: Emerging infectious diseases (Emerg Infect Dis) Vol. 11 Issue 3 Pg. 474-5 (Mar 2005) ISSN: 1080-6040 [Print] United States
• PMID: 15757570 (Publication Type: Journal Article)

Topics

• Adult
• Borrelia (classification, isolation & purification)
• Female
• Humans
• Male
• Polymerase Chain Reaction (methods)
• Relapsing Fever (diagnosis, epidemiology, microbiology)
• Senegal (epidemiology)
• Species Specificity