Use of low toxicity adjuvants and killed virus to induce protective immunity against the Friend murine leukaemia retrovirus-induced disease.

Abstract

Low toxic and synthetic adjuvants were investigated in the induction of protective immunity against Friend murine retrovirus-induced erythroleukaemia by immunization with inactivated Friend murine leukaemia helper virus (F-MuLV). 6-O-(2-tetradecyl-hexadecanoyl)-N-acetylmuramyl-L-alanyl-D-isoglutamine (B30-MDP) showed a significant enhancement of the protective immunity against Friend virus-induced erythroleukaemia not only in H-2a/b mice known to make good immune responses to F-MuLV envelope, but also in H-2a/a mice which are usually unable to respond to F-MuLV envelope protein. Another analogue of N-acetylmuramyl-D-isoglutamine (MDP), N alpha-acetylmuramyl-L-alanyl-D-isoglutaminyl-N epsilon-stearoyl-L-lysine [MDP-Lys(L18)], which has been shown to enhance non-specific protective activity against bacterial and viral infections, however, showed no adjuvant activity in the present system. A combined adjuvant of the synthesized mycobacterial cord factor, trehalose dimycolate (TDM) and detoxified bacterial endotoxin, monophosphoryl lipid A from Salmonella minnesota, gave good protection which was comparable to complete Freund's adjuvant in both H-2a/b and H-2a/a mice.

• Authors: C Ishihara, M Miyazawa, J Nishio, I Azuma, B Chesebro
• Journal: Vaccine (Vaccine) Vol. 10 Issue 5 Pg. 353-6 ( 1992) ISSN: 0264-410X [Print] Netherlands
• PMID: 1574922 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Vaccines, Inactivated
• Viral Vaccines

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Friend murine leukemia virus (immunology)
• Immunization
• Leukemia, Experimental (prevention & control)
• Mice
• Vaccines, Inactivated (immunology)
• Viral Vaccines (immunology)