Abstract | OBJECTIVES: METHODS: GHB intoxication was induced in 20 rats by intraperitoneal injection of 700 mg/kg of the GHB precursor gamma-butyrolactone. One hour later, rats were randomly assigned to receive either physostigmine (0.06 mg/kg) intraperitoneally or an equivalent volume of saline. After administration of physostigmine, rats were continuously monitored by a blinded observer for arousal (return of righting reflex), fasciculations, and seizures. Heart rate and respiratory rate were recorded at 0, 5, 15, and 60 minutes after administration of physostigmine. Data were analyzed using repeated-measures analysis of variance and chi-square test. A pretest sample size calculation determined that 10 rats per group would detect a change in arousal from 0% to 50% and a 10% change in heart rate. RESULTS: No rats in either group had arousal within one hour (p = 1.0); however, ten of ten physostigmine-treated rats developed signs of physostigmine toxicity ( fasciculations, 7; seizures, 3), while no controls developed signs of physostigmine toxicity (p = 0.00). The authors were unable to detect a decrease in heart rate. CONCLUSIONS:
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Authors | Theodore C Bania, Jason Chu |
Journal | Academic emergency medicine : official journal of the Society for Academic Emergency Medicine
(Acad Emerg Med)
Vol. 12
Issue 3
Pg. 185-9
(Mar 2005)
ISSN: 1553-2712 [Electronic] United States |
PMID | 15741579
(Publication Type: Journal Article)
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Chemical References |
- Cholinesterase Inhibitors
- Sodium Oxybate
- Physostigmine
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Topics |
- Animals
- Arousal
(drug effects)
- Cholinesterase Inhibitors
(adverse effects, pharmacology)
- Coma
(chemically induced, drug therapy)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drug Overdose
- Fasciculation
(chemically induced)
- Heart Rate
(drug effects)
- Male
- Physostigmine
(adverse effects, pharmacology)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reflex
(drug effects)
- Respiratory Mechanics
(drug effects)
- Seizures
(chemically induced)
- Sodium Oxybate
(poisoning)
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