Abstract | BACKGROUND: METHODS: The authors explored the effectiveness of a tumor cell vaccine transduced with immune activators, dual-modified using the protein transfer technique. First, a glycosylphosphatidylinositol (GPI)-anchored murine B7.1 (mB7.1-GPI) and a transmembrane-anchored SEA (TM-SEA) were genetically generated. Then, the murine lymphoma EL4 cells were dual modified with the incorporation of mB7.1-GPI and TM-SEA onto the cell surface. Flow cytometry and laser confocal microscopy showed that the incorporation of B7.1 and SEA molecules onto EL4 cells was quite stable. RESULTS: The dual-modified tumor cell vaccine EL4/mB7.1-GPI + TM-SEA elicited significantly stronger antitumor immune responses both in vitro and in vivo when compared with the single-modified tumor cell vaccines EL4/mB7.1-GPI and EL4/TM-SEA. CONCLUSIONS: The results of the current study validated the novel approach for preparing tumor cell vaccines modified with dual immune active molecules using the protein transfer technique, and supported the feasibility and effectiveness of the dual-modified tumor cell vaccine.
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Authors | Pingyong Yi, Hai Yu, Wenxue Ma, Qingqing Wang, Boris R Minev |
Journal | Cancer
(Cancer)
Vol. 103
Issue 7
Pg. 1519-28
(Apr 01 2005)
ISSN: 0008-543X [Print] United States |
PMID | 15739200
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2005 American Cancer Society. |
Chemical References |
- B7-1 Antigen
- Cancer Vaccines
- Enterotoxins
- Glycosylphosphatidylinositols
- enterotoxin A, Staphylococcal
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Topics |
- Animals
- B7-1 Antigen
- Cancer Vaccines
(therapeutic use)
- Cell Membrane
(metabolism)
- Cytotoxicity, Immunologic
- Enterotoxins
(immunology)
- Feasibility Studies
- Female
- Glycosylphosphatidylinositols
- Lymphocyte Activation
- Lymphoma
(immunology, therapy)
- Mice
- Mice, Inbred C57BL
- Neoplasm Transplantation
- Random Allocation
- Transfection
- Tumor Cells, Cultured
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