Abstract | OBJECTIVE: METHODS: Expression of epidermal growth factor receptor (EGFR) in cultured T24 cells was analyzed with Western blot assay. Human bladder cancer T24 cells were exposed to TP40 at 5 - 1 000 microg/L. Methyl thiazolyl tetrazolium assay was applied to evaluate the cell proliferation by measuring the absorbance (A) at 570 nm with a microplate reader. Tritium labeled thymine deoxyriboside ([(3)H]-TdR) uptake was measured to observe DNA synthesis. Competition assays were performed by the EGF at 1 - 7 500 microg/L. RESULTS: Expression of EGFR was high in human bladder cancer T24 cells. Cell growth was suppressed by 10%, 19%, 27%, 41%, 47%, 53% and 61% after 96 h treatment with TP40 at 5, 50, 100, 250, 500, 750 and 1 000 microg/L, respectively. [(3)H]-TdR incorporation was 80%, 69%, 48% and 51% after 24 h, 48 h, 72 h, 96 h treatment with TP40 at 750 microg/L, respectively. When the concentration was 1 - 7 500 microg/L, EGF could block the inhibitory effect of TP40 to some extent. CONCLUSIONS: Human bladder cancer T24 cells express EGFR at a high level. TP40 could inhibit the growth of T24 cells effectively in a dose- and time-dependent manner. The cytotoxic effects of TP40 were specifically mediated by EGFR.
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Authors | Xiang Yan, Qiang Ding, Yuan-fang Zhang, Yong-hua Xu |
Journal | Zhonghua wai ke za zhi [Chinese journal of surgery]
(Zhonghua Wai Ke Za Zhi)
Vol. 42
Issue 23
Pg. 1457-9
(Dec 07 2004)
ISSN: 0529-5815 [Print] China |
PMID | 15733465
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Exotoxins
- Recombinant Fusion Proteins
- Transforming Growth Factor alpha
- transforming growth factor alpha-Pseudomonas exotoxin A (40)
- ErbB Receptors
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Topics |
- Cell Proliferation
(drug effects)
- ErbB Receptors
(metabolism)
- Exotoxins
(pharmacology)
- Humans
- Recombinant Fusion Proteins
(pharmacology)
- Transforming Growth Factor alpha
(pharmacology)
- Tumor Cells, Cultured
- Urinary Bladder Neoplasms
(metabolism, pathology)
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