Abstract | OBJECTIVE:
Protein nitration is a prominent feature of inflammatory processes in the joint. We have developed immunoassays specific for a peptide of the alpha-helical region of type II collagen 108HRGYPGLDG116 (Coll 2-1) and its nitrated form 108HRGY(NO2)PGLDG116 ( Coll 2-1 NO2) in biological fluids. DESIGN: RESULTS: In healthy subjects, Coll 2-1 and Coll 2-1 NO2 concentrations were 125.13+/-3.71 nM and 0.16+/-0.08 nM, respectively. In OA and RA, Coll 2-1 and Coll 2-1 NO2 serum levels were found to be significantly increased compared to controls of the same range of age (Coll 2-1: OA: 200.80+/-8.98 nM, RA: 172.30+/-19.05 nM, normal: 126.60+/-6.70 nM and Coll 2-1 NO2: OA: 0.26+/-0.02, RA: 0.38+/-0.05, normal: 0.12+/-0.01 nM). Coll 2-1 NO2 levels were significantly more elevated in RA than in OA patients (P<0.05). As a consequence, the ratio Coll 2-1 NO2/Coll 2-1 was 1.6 times higher in RA than in OA subjects. No relationship was found between the radiological OA severity and the levels of Coll 2-1 and Coll 2-1 NO2 in serum. Coll 2-1 NO2, but not Coll 2-1, was correlated with C-reactive protein in the sera of OA and RA patients. CONCLUSIONS: The determination of both Coll 2-1 and Coll 2-1 NO2 in serum of arthritic patients seems to be a promising useful tool for the detection of oxidative-related cartilage degradation episode. Further, these markers could be helpful for monitoring the effects of anti-inflammatory or antioxidant drugs on cartilage degradation.
|
Authors | Michelle Deberg, Alain Labasse, Stephan Christgau, Paul Cloos, Dennis Bang Henriksen, Jean-Pierre Chapelle, Brigitte Zegels, Jean-Yves Reginster, Yves Henrotin |
Journal | Osteoarthritis and cartilage
(Osteoarthritis Cartilage)
Vol. 13
Issue 3
Pg. 258-65
(Mar 2005)
ISSN: 1063-4584 [Print] England |
PMID | 15727893
(Publication Type: Journal Article, Validation Study)
|
Chemical References |
- Biomarkers
- Collagen Type II
- Free Radicals
- Immune Sera
- Nitrates
- Peptide Fragments
|
Topics |
- Adult
- Aging
(blood)
- Animals
- Antibody Specificity
- Arthritis, Rheumatoid
(blood, metabolism)
- Biomarkers
(blood)
- Collagen Type II
(blood, immunology)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Free Radicals
(blood)
- Humans
- Immune Sera
(immunology)
- Middle Aged
- Nitrates
(blood)
- Osteoarthritis
(blood, metabolism)
- Oxidation-Reduction
- Peptide Fragments
(blood)
- Rabbits
- Reproducibility of Results
|