Chronological changes of neurofilament 200 kDa immunoreactivity in the lateral olfactory tract after transient forebrain ischemia in gerbils.

Abstract

This study was carried out to investigate the transient ischemia-induced changes of neurofilament 200 kDa (NF-200) immunoreactivity and protein content in the gerbil lateral olfactory tract (LOT) after 5 min of transient forebrain ischemia. Weak NF-200 immunoreactivity was detectable in the LOT in the sham-operated group. In this group, a few somata of mitral cells showed weak NF-200 immunoreactivity. One day after transient ischemia, NF-200 immunoreactivity in the LOT was increased significantly. NF-200 immunoreactivity in the LOT by 15 days after ischemia was similar to that in the 1 day post-ischemic group. In this time period, strong NF-200 immunoreactivity was expressed in the mitral cell processes, but the immunoreactivity in the mitral cell somata was significantly decreased. Thereafter, NF-200 immunoreactivity in the LOT was decreased significantly by 30 days after ischemic insult. At this time after ischemia, NF-200 immunoreactivity in the mitral cell dendrites was significantly decreased. The result of Western blot study showed that the pattern of NF-200 expression was similar to that of immunohistochemistry after ischemia-reperfusion. Our result suggests that changes of NF-200 protein in the gerbil LOT may be related to response to ischemic damage and that the axonal transport followed transient ischemia may be disturbed.

• Authors: Hyeon Yong Lee, In Koo Hwang, Un San Koh, Jae Chul Lee, Dae Ho Kim, Jung Hwa Kim, Chang Ho Kim, Ki-Yeon Yoo, Tae-Cheon Kang, Baek Hwang, Moo Ho Won
• Journal: Brain research (Brain Res) Vol. 1036 Issue 1-2 Pg. 202-7 (Mar 02 2005) ISSN: 0006-8993 [Print] Netherlands
• PMID: 15725419 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neurofilament Proteins
• neurofilament protein H

Topics

• Animals
• Axonal Transport (physiology)
• Axons (metabolism)
• Cytoskeleton (metabolism)
• Dendrites (metabolism)
• Disease Models, Animal
• Gerbillinae
• Immunohistochemistry
• Ischemic Attack, Transient (metabolism, physiopathology)
• Male
• Neurofilament Proteins (metabolism)
• Olfactory Bulb (metabolism, physiopathology)
• Olfactory Pathways (metabolism, physiopathology)
• Time Factors
• Up-Regulation (physiology)