Unclosed beta-propellers display stable structures: implications for substrate access to the active site of prolyl oligopeptidase.

Abstract

Prolyl oligopeptidase is implicated in the metabolism of neuropeptides and is involved in amnesia and depression. It contains a peptidase and an unusual beta-propeller domain that excludes large peptides and proteins from the active site. The propeller consists of seven blades not closed by a "Velcro" between the first and last blades. The propeller domain was expressed as a stable, soluble protein, P(7). Its conformational identity with that of the native propeller was verified by circular dichroism and digestion with trypsin. Differential scanning calorimetry, kinetic denaturation with urea and equilibrium denaturation with guanidinium chloride have shown that the propeller is more stable than the parent prolyl oligopeptidase. The deletion of the seventh blade of P(7) led to a stable structure, a six-bladed propeller, P(6), which immediately dimerized, in contrast with the monomeric P(7). Addition of an 11 amino acid residue extension to the C terminus of P(6) also produced a dimer, whereas the P(6) labelled with a His-tag at the N terminus displayed a monomer structure. The stability of P(6) and its variants was lower than that of P(7). The denatured propellers refolded readily. This study shows that the unclosed P(7) is a stable structure, and suggests that an opening between the peptidase and the propeller domains is more important for the substrate entry than is the putative opening between the first and seventh blades. Our results suggest that the propellers are simple, versatile structures, which can be prepared artificially.

• Authors: Tünde Juhász, Zoltán Szeltner, Vilmos Fülöp, László Polgár
• Journal: Journal of molecular biology (J Mol Biol) Vol. 346 Issue 3 Pg. 907-17 (Feb 25 2005) ISSN: 0022-2836 [Print] Netherlands
• PMID: 15713471 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anilino Naphthalenesulfonates
• Peptide Fragments
• Recombinant Proteins
• 1-anilino-8-naphthalenesulfonate
• Serine Endopeptidases
• Prolyl Oligopeptidases

Topics

• Anilino Naphthalenesulfonates (metabolism)
• Animals
• Calorimetry, Differential Scanning
• Catalytic Domain
• Circular Dichroism
• Dimerization
• Enzyme Stability
• In Vitro Techniques
• Models, Molecular
• Mutagenesis, Site-Directed
• Peptide Fragments (chemistry, genetics, metabolism)
• Prolyl Oligopeptidases
• Protein Denaturation
• Protein Folding
• Protein Structure, Tertiary
• Recombinant Proteins (chemistry, genetics, metabolism)
• Serine Endopeptidases (chemistry, genetics, metabolism)
• Solubility
• Substrate Specificity
• Swine
• Thermodynamics