Blood-brain barrier disruption and lesion localisation in experimental autoimmune encephalomyelitis with predominant cerebellar and brainstem involvement.

Abstract

The role of the blood-brain barrier (BBB) in determining lesion distribution was assessed in an atypical model of experimental autoimmune encephalomyelitis (EAE) induced in C3H/HeJ mice by immunisation with peptide 190-209 of myelin proteolipid protein, which can result in two distinct types of EAE, each with distinct lesion distribution. Areas of the BBB showing constitutively greater permeability in naive mice did not correlate with the lesion distribution in EAE. BBB disruption occurred only in sites of inflammatory cell infiltration. Irrespective of the clinical type, the BBB was disrupted in the cerebellum and brainstem. Pertussis toxin had no effect on lesion distribution. Thus, lesion distribution is not influenced solely by BBB permeability.

• Authors: Diane M Muller, Michael P Pender, Judith M Greer
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 160 Issue 1-2 Pg. 162-9 (Mar 2005) ISSN: 0165-5728 [Print] Netherlands
• PMID: 15710469 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Horseradish Peroxidase
• Peroxidase
• Pertussis Toxin

Topics

• Amino Acid Sequence
• Animals
• Biomarkers (analysis)
• Blood-Brain Barrier (immunology)
• Brain Stem (enzymology, pathology, physiopathology)
• Capillary Permeability (immunology)
• Cerebellum (enzymology, pathology, physiopathology)
• Encephalomyelitis, Autoimmune, Experimental (enzymology, pathology, physiopathology)
• Female
• Horseradish Peroxidase (administration & dosage, analysis)
• Infusions, Intravenous
• Mice
• Mice, Inbred C3H
• Molecular Sequence Data
• Peroxidase (metabolism)
• Pertussis Toxin (administration & dosage)
• Spinal Cord (enzymology, pathology)
• Staining and Labeling