Flavonoids from the leaves of Diospyros kaki reduce hydrogen peroxide-induced injury of NG108-15 cells.

Abstract

Flavonoids from the leaves of Diospyros kaki L (FLDK-P70) are main therapeutic components of NaoXingQing, which is a novel and patented Traditional Chinese Medicine drug used for the treatment of syndrome of apoplexy for years in China. The present study investigated the effects of FLDK-P70 on hydrogen peroxide (H2O2)-induced apoptosis-like damage of NG108-15 cells. Pretreatment of cells with FLDK-P70 alleviated H2O2-induced cell injury and apoptosis by upregulating Bcl-2 expression and improving redox imbalance as indicated by the alleviation of the decline in the intracellular endogenous antioxidants: glutathione and glutathione peroxidase as well as catalase, with the decrease of the leak of lactate dehydrogenase and the reduction of the accumulation of malondialdehyde. These results indicate that FLDK-P70 may be potentially used in the prevention and treatment of ischemia/reperfusion injury and other neurodegenerative disease. Upregulating bcl-2 and improving cellular redox state by FLDK-P70 may play critical roles in attenuating oxidative injury.

• Authors: Weijian Bei, Wenlie Peng, Yan Ma, Anlong Xu
• Journal: Life sciences (Life Sci) Vol. 76 Issue 17 Pg. 1975-88 (Mar 11 2005) ISSN: 0024-3205 [Print] Netherlands
• PMID: 15707880 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Flavonoids
• Proto-Oncogene Proteins c-bcl-2
• Hydrogen Peroxide

Topics

• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Cell Survival (drug effects)
• Diospyros (chemistry)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Flavonoids (pharmacology)
• Humans
• Hydrogen Peroxide (pharmacology)
• Medicine, Chinese Traditional
• Neuroblastoma (drug therapy, pathology)
• Oxidation-Reduction
• Oxidative Stress (drug effects)
• Plant Leaves (chemistry)
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
• Tumor Cells, Cultured (drug effects)
• Up-Regulation