[Protective effects of acidic fibroblast growth factor on intestinal ischemia/reperfusion in rats].

Abstract

OBJECTIVE:

To observe the change in hepatic and renal functions, change in the plasma D-lactate level, and the expression of proliferating cell nuclear antigen (PCNA) after intestinal I/R injury, so as to explore the effects of reconstructive human acid fibroblast growth factor(aFGF) on intestinal I/R injury in rats.

METHODS:

One hundred and twenty-six Wistar rats were divided into sham-operated, ischemia (45 minutes) plus reperfusion, reconstructive human aFGF treatment (2, 4, 8 microg aFGF) and wild type aFGF(2, 6, 12, and 24 hours, respectively) groups. Hepatic and renal functions and the levels of plasma D-lactate were determined and the expression of PCNA was assessed.

RESULTS:

Compared with all other groups, bowel barrier function and hepatic and renal functions showed most marked deterioration in sham-operated group. The damages were less marked in reconstructive human aFGF group compared with other groups 24 hours after ischemia/reperfusion of the intestine, and the protective effect was best shown when 4 microg of aFGF was given. The trend of expression of PCNA was similar to that of changes in D-lactate level.

CONCLUSION:

Wild type reconstructive human aFGF treatment significantly improves the outcome of ischemia/reperfusion injury to the intestine, and the effect is dose-dependent.

• Authors: Li-xin Weng, Xiao-bing Fu, Xiu-xia Li, Tong-zhu Sun, Shu-yun Zheng, Wei Chen, Jun-you Li
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 2 Pg. 98-101 (Feb 2005) ISSN: 1003-0603 [Print] China
• PMID: 15698495 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proliferating Cell Nuclear Antigen
• Fibroblast Growth Factor 1
• Lactic Acid

Topics

• Animals
• Disease Models, Animal
• Fibroblast Growth Factor 1 (pharmacology)
• Humans
• Intestines (blood supply, pathology)
• Lactic Acid (blood)
• Male
• Proliferating Cell Nuclear Antigen (metabolism)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism, pathology, prevention & control)