Pineapple juice for digestion of swamp eel viscera for harvesting infective-stage larva of Gnathostoma spp.

Abstract

Third-stage larvae were used as antigen in the diagnosis of gnathostomiasis in Western blot analysis. Normally, the larvae were obtained from digestion of eel's liver (Fluta alba) by the enzyme pepsin. We used pineapple juice (Ananus comosus) instead of enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. The difference in recovered larvae numbers, between pineapple juice and pepsin, were not statistically significantly different (p>0.05). The larvae from pepsin and pineapple juice digestion were cultivated on BME for 7 days; the survival rates were not significantly different (p>0.05). Thus, pineapple juice is another enzyme of choice for recovering Gnathostoma spinigerum third-stage larvae.

• Authors: Suphan Soogarun, Jamsai Suwansaksri, Viroj Wiwanitkit
• Journal: The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health) Vol. 35 Issue 2 Pg. 267-9 (Jun 2004) ISSN: 0125-1562 [Print] Thailand
• PMID: 15691122 (Publication Type: Journal Article)

Chemical References

• Antigens, Helminth
• Solutions
• Pepsin A

Topics

• Ananas (metabolism)
• Animals
• Antigens, Helminth
• Beverages
• Blotting, Western
• Digestion (physiology)
• Eels (parasitology)
• Fish Diseases (diagnosis, parasitology)
• Food Parasitology
• Gnathostoma (immunology, isolation & purification)
• Larva (immunology, parasitology)
• Larva Migrans, Visceral
• Liver (parasitology)
• Pepsin A (metabolism)
• Solutions
• Spirurida Infections (diagnosis)