Factors influencing axillary shoot proliferation and adventitious budding in cedar.

Abstract

We developed procedures for in vitro cloning of Cedrus atlantica Manetti and C. libani A. Rich explants from juvenile and mature plants. Explant size was one determinant of the frequency of axillary bud break in both species. Shoot tips and nodal explants mainly developed calli, whereas bud sprouting occurred in defoliated microcuttings cultured on a modified Murashige and Skoog medium without growth regulators. Isolation and continuous subculture of sprouted buds on the same medium allowed cloning of microcuttings from C. atlantica and C. libani seedlings and bicentennial C. libani trees, thus providing a desirable alternative for multiplying mature trees that have demonstrated superior characteristics. We also report adventitious bud differentiation from isolated embryos of C. atlantica. Neither auxin treatments nor other methods tested, including infection with Agrobacterium rhizogenes, were effective in inducing root initiation.

• Authors: Begoña Renau-Morata, Javier Ollero, Isabel Arrillaga, Juan Segura
• Journal: Tree physiology (Tree Physiol) Vol. 25 Issue 4 Pg. 477-86 (Apr 2005) ISSN: 0829-318X [Print] Canada
• PMID: 15687096 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Growth Regulators

Topics

• Cedrus (growth & development)
• Plant Growth Regulators
• Plant Roots (growth & development)
• Plant Shoots (genetics)
• Seeds (growth & development)
• Temperature
• Tissue Culture Techniques