HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mechanisms underlying anorexia after microinjection of bombesin into the lateral cerebroventricle.

Abstract
Intracerebroventricular (i.c.v.) injections of bombesin (BN) and gastrin-releasing peptide (GRP) dose-dependently decreased food intake in male Wistar rats fasted for 17 h. Neuromedin B (NMB) did not show any effect on food intake. After BN administration, locomotor activity did not significantly change, compared with a vehicle-injected group. The anorexia induced by BN (0.3 microg) was perfectly inhibited by pretreatment with a GRP-receptor antagonist, [D-Tyr(6)]BN(6-13) methyl ester (10 microg), an NO synthase inhibitor, L-nitro-arginine (30 microg), and a PKG inhibitor, H-9 (2 microg). The cGMP concentration in the hypothalamus increased 1 h after administration when compared with the vehicle-injected group. On the other hand, an NMB-receptor antagonist, BIM23127 (10 microg), and the protein kinase (PK) C inhibitors, chelerythrine (2 microg) and Go6983 (2 microg), inhibited only the late phase of the anorexia. A PKC activator, phorbol 12, 13-dibutyrate (3 microg), injected into the ventricle decreased food intake. These findings suggest that BN suppresses food intake mainly mediated through the GRP receptor and NO-cGMP-PKG pathway, and NMB receptor and PKC is partly involved in the late phase of the anorexia.
AuthorsHiromi Tsushima, Mayumi Mori
JournalPharmacology, biochemistry, and behavior (Pharmacol Biochem Behav) Vol. 80 Issue 2 Pg. 289-96 (Feb 2005) ISSN: 0091-3057 [Print] United States
PMID15680182 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Gastrin-Releasing Peptide
  • Bombesin
Topics
  • Animals
  • Anorexia (chemically induced)
  • Bombesin (administration & dosage)
  • Eating (drug effects, physiology)
  • Gastrin-Releasing Peptide (administration & dosage)
  • Lateral Ventricles (drug effects, physiology)
  • Male
  • Microinjections (methods)
  • Motor Activity (drug effects, physiology)
  • Rats
  • Rats, Wistar
  • Telencephalon (drug effects, physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: