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Effects of postmortem heparinization in canine lung transplantation with non-heart-beating donors.

AbstractOBJECTIVE:
Microthrombus formation appears to be one of the major detrimental factors in lung transplantation from non-heart-beating donors. The purpose of this study was to evaluate the effects of postmortem heparinization by closed-chest cardiac massage in a canine model of left single-lung allotransplantation from non-heart-beating donors.
METHODS:
Left lung transplantation was performed in 18 weight-matched pairs of mongrel dogs. Donors were killed with an intravenous injection of potassium chloride and left at room temperature for 2 hours. The cadaveric donors were assigned randomly to one of the three groups. In group 1 (n = 6), no heparin was given as a control. In group 2 (n = 6), heparin sodium (1000 U/kg) was administered intravenously before cardiac arrest. In group 3 (n = 6), heparin sodium (1000 U/kg) was administered intravenously 10 minutes after death, then closed-chest cardiac massage was performed for 2 minutes. After 2 hours of cardiac arrest, donor lungs were flushed with low-potassium dextran-glucose solution and preserved for 60 minutes. After left lung transplantation, the right pulmonary artery was ligated, and recipient animals were followed up for 3 hours. Univariate and multivariate repeated analyses were used for statistics.
RESULTS:
Both groups 2 and 3 had significantly better gas exchange and lower pulmonary vascular resistance than group 1. Changes in thrombin-antithrombin III complex concentration during the warm ischemia indicated that postmortem heparinization suppressed clotting activation in the donor.
CONCLUSIONS:
Postmortem heparinization by cardiac massage is beneficial in lung transplantation from non-heart beating donors by preventing microthrombus formation.
AuthorsHidetoshi Inokawa, Hiroshi Date, Mikio Okazaki, Daisuke Okutani, Keiju Aokage, Itaru Nagahiro, Motoi Aoe, Yoshifumi Sano, Nobuyoshi Shimizu
JournalThe Journal of thoracic and cardiovascular surgery (J Thorac Cardiovasc Surg) Vol. 129 Issue 2 Pg. 429-34 (Feb 2005) ISSN: 0022-5223 [Print] United States
PMID15678056 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anticoagulants
  • Biomarkers
  • antithrombin III-protease complex
  • Antithrombin III
  • Heparin
  • Peptide Hydrolases
Topics
  • Animals
  • Anticoagulants (pharmacology)
  • Antithrombin III (drug effects, metabolism)
  • Biomarkers (blood)
  • Disease Models, Animal
  • Dogs
  • Heart (drug effects, physiopathology)
  • Heart Arrest, Induced
  • Heart Massage
  • Heparin (pharmacology)
  • Lung (drug effects, metabolism, pathology)
  • Lung Transplantation
  • Models, Cardiovascular
  • Peptide Hydrolases (drug effects, metabolism)
  • Postmortem Changes
  • Respiratory Function Tests
  • Vascular Resistance (drug effects, physiology)
  • Whole Blood Coagulation Time

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