Musk xylene is a novel specific inducer of cytochrome P-450IA2.

Abstract

The effect of musk xylene on contents of both cytochrome P-450IA1 and cytochrome P-450IA2 in rat liver was investigated using Western blotting analysis. Rats were treated i.p. for five consecutive days with either 50, 100 or 200 mg musk xylene/kg body weight. Musk xylene increased both total cytochrome P-450 and cytochrome b5 contents in rat liver microsomes. Musk xylene induced cytochrome P-450IA2 (384 pmol/mg protein) strongly and preferentially and the ratio of cytochrome P450IA2/P-450IA1 was about 12 at the lowest dose tested. Musk xylene also induced the cytochrome P-450IA1 dose-dependently, but these extents were very small (32-174 pmol/mg protein). These results suggest that musk xylene may be a more specific inducer for cytochrome P-450IA2 than any other inducers reported.

• Authors: N Iwata, K Minegishi, K Suzuki, Y Ohno, T Kawanishi, A Takahashi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 184 Issue 1 Pg. 149-53 (Apr 15 1992) ISSN: 0006-291X [Print] United States
• PMID: 1567420 (Publication Type: Journal Article)

Chemical References

• Perfume
• Xylenes
• musk xylene
• Cytochromes b5
• Cytochrome P-450 Enzyme System
• Oxidoreductases
• Cytochrome P-450 CYP1A2

Topics

• Animals
• Blotting, Western
• Cytochrome P-450 CYP1A2
• Cytochrome P-450 Enzyme System (biosynthesis)
• Cytochromes b5 (biosynthesis)
• Dose-Response Relationship, Drug
• Enzyme Induction
• Kinetics
• Male
• Microsomes, Liver (drug effects, metabolism)
• Oxidoreductases (biosynthesis)
• Perfume
• Rats
• Rats, Inbred Strains
• Reference Values
• Xylenes (pharmacology)