Abstract |
The purpose of this study was to investigate the potential neuroprotective efficacy of the mitochondrial uncoupler 2,4-dinitrophenol (DNP) in rats following a mild to moderate spinal cord contusion injury. Animals received intraperitoneal injections of vehicle ( DMSO) or 5 mg/mL of DNP prior to injury. Twenty-four hours following surgery, mitochondrial function was assessed in mitochondria isolated from spinal cord synaptosomes. In addition, synaptosomes were used to measure indicators of reactive oxygen species formation, lipid peroxidation, and protein oxidation. Relative to vehicle-treated animals, pretreatment with DNP maintained mitochondrial bioenergetics and significantly decreased reactive oxygen species levels, lipid peroxidation, and protein carbonyl content following spinal cord injury. Furthermore, pretreatment with DNP significantly increased the amount of remaining white matter at the injury epicenter 6 weeks after injury. These results indicate that treatment with mitochondrial uncoupling agents may provide a novel approach for the treatment of secondary injury following spinal cord contusion.
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Authors | Ying Jin, Melanie L McEwen, Stephanie A Nottingham, William F Maragos, Natasha B Dragicevic, Patrick G Sullivan, Joe E Springer |
Journal | Journal of neurotrauma
(J Neurotrauma)
Vol. 21
Issue 10
Pg. 1396-404
(Oct 2004)
ISSN: 0897-7151 [Print] United States |
PMID | 15672630
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Reactive Oxygen Species
- Uncoupling Agents
- 2,4-Dinitrophenol
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Topics |
- 2,4-Dinitrophenol
(pharmacology)
- Animals
- Disease Models, Animal
- Female
- Lipid Peroxidation
(drug effects)
- Mitochondria
(drug effects)
- Oxidative Stress
(drug effects)
- Rats
- Rats, Long-Evans
- Reactive Oxygen Species
(metabolism)
- Spinal Cord Injuries
(pathology, prevention & control)
- Uncoupling Agents
(pharmacology)
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