Abstract | BACKGROUND:
Interleukin (IL) 7 is known to stimulate growth of breast cancer cells in vitro. It has been recently associated with node-positive tumours and with poor survival in breast cancer. The effects of IL-7 on the lymphangiogenic properties of breast cancer cells were explored. METHODS: RESULTS: All VEGFs ( VEGF-A, -B, -C and -D) were expressed in breast cancer cells, but at different levels. IL-7 increased the expression of VEGF-D at both mRNA and protein levels in MCF-7 and MDA MB-231 cells. In the in vivo model, IL-7 significantly induced the formation of lymphatic LYVE-1-positive, but not vascular von Willebrand factor-positive, microtubules (P = 0.021 versus sections without IL-7). CONCLUSION:
IL-7 induced the lymphangiogenic properties of breast cancer cells, probably by upregulation of VEGF-D. This might have a significant impact on the lymphatic spread of breast cancer.
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Authors | M A A Al-Rawi, G Watkins, R E Mansel, W G Jiang |
Journal | The British journal of surgery
(Br J Surg)
Vol. 92
Issue 3
Pg. 305-10
(Mar 2005)
ISSN: 0007-1323 [Print] England |
PMID | 15672426
(Publication Type: Journal Article)
|
Chemical References |
- Interleukin-7
- Neoplasm Proteins
- RNA, Messenger
- Vascular Endothelial Growth Factor D
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Topics |
- Animals
- Blotting, Western
- Breast Neoplasms
(metabolism)
- Cell Line, Tumor
(drug effects)
- Female
- Humans
- Interleukin-7
(pharmacology)
- Lymphangiogenesis
(physiology)
- Mice
- Mice, Nude
- Neoplasm Proteins
(metabolism)
- Neoplasm Transplantation
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Up-Regulation
- Vascular Endothelial Growth Factor D
(metabolism)
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