Mode of action of cellulases on dyed cotton with a reactive dye.

Cotton woven fabrics which were previously dyed with a reactive dye were treated with a commercial cellulase preparation. Dyeing with a reactive dye for cotton apparently inhibited the weight loss activity and saccharification activity of cellulase. In addition, dyed cotton was treated with highly purified cellulases which were exo-type cellulases (Cellobiohydrolase I (CBH I) and Cellobiohydrolase II (CBH II)) and endo-type cellulase (Endoglucanase II (EG II)). Exo-type cellulases were inhibited more than endo-type cellulase by dyeing in the case of saccharification activity. CBH I was severely inhibited by dyeing as compared with CBH II or EG II from the viewpoint of morphological changes in the fiber surface. Dyes on the cellulose substrates severely influenced CBH I in spite of the rare modification, because CBH I hydrolyzed cellulose with true-processive action. The change in the activity of each cellulase component on dyed cotton can affect the synergistic action of cellulases.
AuthorsMinoru Yamada, Yoshihiko Amano, Eisuke Horikawa, Kouichi Nozaki, Takahisa Kanda
JournalBioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 69 Issue 1 Pg. 45-50 (Jan 2005) ISSN: 0916-8451 [Print] Japan
PMID15665466 (Publication Type: Journal Article)
Chemical References
  • Coloring Agents
  • endoglucanase 2
  • Cellulase
  • Cellulose 1,4-beta-Cellobiosidase
  • Cellulase (chemistry, metabolism)
  • Cellulose 1,4-beta-Cellobiosidase (chemistry, metabolism)
  • Coloring Agents (chemistry)
  • Cotton Fiber
  • Textile Industry (methods)

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