Tumor recognition following Vgamma9Vdelta2 T cell receptor interactions with a surface F1-ATPase-related structure and apolipoprotein A-I.

Abstract

Vgamma9Vdelta2 T lymphocytes, a major gammadelta T lymphocyte subset in humans, display cytolytic activity against various tumor cells upon recognition of yet uncharacterized structures. Here, we show that an entity related to the mitochondrial F1-ATPase is expressed on tumor cell surface and promotes tumor recognition by Vgamma9Vdelta2 T cells. When immobilized, purified F1-ATPase induces selective activation of this lymphocyte subset. The Vgamma9Vdelta2 T cell receptors (TCR) and the F1-ATPase also bind a delipidated form of apolipoprotein A-I (apo A-I), as demonstrated by surface plasmon resonance. Moreover, the presence of apo A-I in the culture medium is required for optimal activation of Vgamma9Vdelta2 T cells by tumors expressing F1-ATPase. This study thus describes an unanticipated tumor recognition mechanism by Vgamma9Vdelta2 lymphocytes and a possible link between gammadelta T cell immunity and lipid metabolism.

• Authors: Emmanuel Scotet, Laurent O Martinez, Ethan Grant, Ronald Barbaras, Paul Jenö, Martine Guiraud, Bernard Monsarrat, Xavier Saulquin, Sophie Maillet, Jean-Pierre Estève, Frédéric Lopez, Bertrand Perret, Xavier Collet, Marc Bonneville, Eric Champagne
• Journal: Immunity (Immunity) Vol. 22 Issue 1 Pg. 71-80 (Jan 2005) ISSN: 1074-7613 [Print] United States
• PMID: 15664160 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein A-I
• Ligands
• Receptors, Antigen, T-Cell
• Receptors, Antigen, T-Cell, gamma-delta
• Proton-Translocating ATPases

Topics

• Apolipoprotein A-I (metabolism)
• Humans
• Immobilization
• Jurkat Cells
• Kinetics
• Ligands
• Lymphocyte Activation
• Neoplasms (immunology)
• Protein Binding
• Proton-Translocating ATPases (metabolism)
• Receptors, Antigen, T-Cell (metabolism)
• Receptors, Antigen, T-Cell, gamma-delta (immunology, metabolism)
• Surface Plasmon Resonance
• Surface Properties
• T-Lymphocyte Subsets (immunology, metabolism)
• Tumor Cells, Cultured