Remedial effect of DL-alpha-lipoic acid against adriamycin induced testicular lipid peroxidation.

Abstract

Adriamycin (ADR), a cytotoxic antineoplastic drug is used in the treatment of various solid tumors. However, its efficacy continues to be challenged by significant toxicities including testicular toxicity. In the present study, the effect of lipoic acid, a "universal antioxidant" was investigated on ADR induced peroxidative damages in rat testis. Adult male albino rats of Wistar strain were administered ADR (1 mg/kg body weight, i.v.) once a week for 10 weeks. ADR injected rats showed a significant decline in the activities of enzymic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase) and non-enzymic antioxidants (reduced glutathione, Vitamin A, Vitamin C and Vitamin E) with high malondialdehyde levels. The extent of testicular toxicity was evident from the decreased activities of testicular marker enzymes (sorbitol dehydrogenase and glucose-6-phosphate dehydrogenase). Treatment with lipoic acid (35 mg/kg body weight, i.p.) one day prior to ADR administration, maintained near normal activities of the enzymes and significantly reduced lipid peroxidation, thereby proving it to be an effective cytoprotectant.

• Authors: Chidambaram Prahalathan, Elangovan Selvakumar, Palaninathan Varalakshmi
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 267 Issue 1-2 Pg. 209-14 (Dec 2004) ISSN: 0300-8177 [Print] Netherlands
• PMID: 15663203 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Antioxidants
• Enzymes
• Protective Agents
• Malondialdehyde
• Thioctic Acid
• Doxorubicin
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• Glutathione Reductase
• Glutathione Transferase
• Glutathione

Topics

• Animals
• Antibiotics, Antineoplastic (adverse effects)
• Antioxidants (administration & dosage, pharmacology)
• Catalase (drug effects)
• Doxorubicin (adverse effects)
• Enzymes (drug effects, metabolism)
• Glutathione (drug effects)
• Glutathione Peroxidase (drug effects)
• Glutathione Reductase (drug effects)
• Glutathione Transferase (drug effects)
• Injections, Intravenous
• Lipid Peroxidation (drug effects)
• Male
• Malondialdehyde (metabolism)
• Protective Agents (administration & dosage, pharmacology)
• Rats
• Rats, Wistar
• Superoxide Dismutase (drug effects)
• Testis (drug effects, enzymology, pathology)
• Thioctic Acid (administration & dosage, pharmacology)