Abstract | PURPOSE: METHODS: Twenty multiple intestinal neoplasia mice, heterozygous for the human homologue adenomatous polyposis coli gene, were randomly assigned to three groups: no treatment (n = 8); control plasmid containing green fluorescence protein reporter gene (n = 6); and plasmid containing the full-length adenomatous polyposis coli gene (n = 6). For the adenomatous polyposis coli-treated and green fluorescence protein reporter gene-treated groups, each mouse received the appropriate plasmid complexed with liposome, administered twice per week by oral gavage regime. Treatment lasted four weeks and all animals were killed at the end of treatment period with harvesting of intestinal tissue for polyp number estimation. RESULTS: There was a statistically significant 25 percent reduction in the total number of polyps in the adenomatous polyposis coli-treated (73.1 +/- 1.4) group compared with untreated control (97.8 +/- 5.3, P < 0.01, Tukey test) and multiple intestinal neoplasia mice treated with control green fluorescence protein gene (103.3 +/- 1.7, P < 0.01, Tukey test). CONCLUSION:
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Authors | Jack Lee, Rachel Hargest, Harpreet Wasan, Robin K S Phillips |
Journal | Diseases of the colon and rectum
(Dis Colon Rectum)
Vol. 47
Issue 12
Pg. 2105-13
(Dec 2004)
ISSN: 0012-3706 [Print] United States |
PMID | 15657662
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipids
- Lipofectamine
- Green Fluorescent Proteins
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Topics |
- Adenomatous Polyposis Coli
(genetics, therapy)
- Administration, Oral
- Analysis of Variance
- Animals
- Disease Models, Animal
- Enteral Nutrition
- Gene Transfer Techniques
(standards)
- Genes, APC
- Genes, Reporter
(genetics)
- Genetic Therapy
(methods, standards)
- Germ-Line Mutation
(genetics)
- Green Fluorescent Proteins
(pharmacokinetics, therapeutic use)
- Humans
- Intestinal Neoplasms
(genetics, therapy)
- Lipids
(pharmacokinetics, therapeutic use)
- Mice
- Mice, Inbred C57BL
- Plasmids
(pharmacokinetics, therapeutic use)
- Random Allocation
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
- Tissue Distribution
- Treatment Outcome
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