Abstract |
6-methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 microM on causing apoptotic cell death in HT29 colon carcinoma cells (IC50 = 5.0+/-0.2 microM). Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.
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Authors | Yong-Jin Lee, Hu-Quan Yin, Young-Ho Kim, Guang-Yong Li, Byung-Hoon Lee |
Journal | Archives of pharmacal research
(Arch Pharm Res)
Vol. 27
Issue 12
Pg. 1253-7
(Dec 2004)
ISSN: 0253-6269 [Print] Korea (South) |
PMID | 15646800
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 6-methoxydihydrosanguinarine
- Alkaloids
- Antineoplastic Agents, Phytogenic
- Benzophenanthridines
- Growth Inhibitors
- Isoquinolines
- Phenanthridines
- Plant Extracts
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Topics |
- Alkaloids
(chemistry, isolation & purification, pharmacology)
- Antineoplastic Agents, Phytogenic
(chemistry, isolation & purification, pharmacology)
- Apoptosis
(drug effects, physiology)
- Benzophenanthridines
- Cell Proliferation
(drug effects)
- Colonic Neoplasms
(drug therapy, pathology)
- Colorectal Neoplasms
(drug therapy, pathology)
- Growth Inhibitors
(chemistry, isolation & purification, pharmacology)
- HT29 Cells
- Humans
- Isoquinolines
- Phenanthridines
(chemistry, isolation & purification, pharmacology)
- Plant Extracts
(chemistry, isolation & purification, pharmacology)
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