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Apoptosis inducing effects of 6-methoxydihydrosanguinarine in HT29 colon carcinoma cells.

Abstract
6-methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 microM on causing apoptotic cell death in HT29 colon carcinoma cells (IC50 = 5.0+/-0.2 microM). Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.
AuthorsYong-Jin Lee, Hu-Quan Yin, Young-Ho Kim, Guang-Yong Li, Byung-Hoon Lee
JournalArchives of pharmacal research (Arch Pharm Res) Vol. 27 Issue 12 Pg. 1253-7 (Dec 2004) ISSN: 0253-6269 [Print] Korea (South)
PMID15646800 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 6-methoxydihydrosanguinarine
  • Alkaloids
  • Antineoplastic Agents, Phytogenic
  • Benzophenanthridines
  • Growth Inhibitors
  • Isoquinolines
  • Phenanthridines
  • Plant Extracts
Topics
  • Alkaloids (chemistry, isolation & purification, pharmacology)
  • Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
  • Apoptosis (drug effects, physiology)
  • Benzophenanthridines
  • Cell Proliferation (drug effects)
  • Colonic Neoplasms (drug therapy, pathology)
  • Colorectal Neoplasms (drug therapy, pathology)
  • Growth Inhibitors (chemistry, isolation & purification, pharmacology)
  • HT29 Cells
  • Humans
  • Isoquinolines
  • Phenanthridines (chemistry, isolation & purification, pharmacology)
  • Plant Extracts (chemistry, isolation & purification, pharmacology)

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