High antitumor activity using intratumoral injection of plasmid DNA with mutant-type p27Kip1 gene following in vivo electroporation.

Abstract	In this study, we attempted to use a non-viral gene transfer system, in vivo electroporation, in oral cancer cell B88 xenografts. To evaluate this in vivo gene transfer method, the GFP gene was transfected into xenografts by electroporation. Then, the efficiency of transfection of exogenous p27Kip1 gene by electroporation was confirmed by Western blot analysis. Next, to estimate the reduction of oral cancer xenografts by this method, we measured the size of B88 xenografts in nude mice after electroporation with the wild- or mutant-type p27Kip1 gene. The growth of tumors was markedly suppressed by mutant-type p27Kip1 gene transfection by electroporation compared with transfection of wild-type p27Kip1 gene or empty vector only. Moreover, histological specimens revealed apoptotic cell death was increased in mutant-type p27Kip1-transfected tumors compared to wild-type or empty vector only. These results suggest that it is possible to transfer mutant-type p27Kip1 into oral cancer xenografts using electroporation and to suppress the growth of tumors, furthermore, it is suggested that this system might be used for oral cancer.
Authors	Koji Harada, Supriatno, Shin-Ichi Kawaguchi, Tomitaro Onoue, Yuichiro Kawashima, Hideo Yoshida, Mitsunobu Sato
Journal	Oncology reports (Oncol Rep) Vol. 13 Issue 2 Pg. 201-6 (Feb 2005) ISSN: 1021-335X [Print] Greece
PMID	15645112 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Cdkn1b protein, mouse Cell Cycle Proteins Tumor Suppressor Proteins Cyclin-Dependent Kinase Inhibitor p27
Topics	Animals Cell Cycle Proteins (genetics) Cyclin-Dependent Kinase Inhibitor p27 Electroporation Gene Transfer Techniques Genetic Therapy Genetic Vectors Humans Injections Male Mice Mice, Nude Mouth Neoplasms (therapy) Neoplasm Transplantation Neoplasms, Squamous Cell (therapy) Plasmids Transfection Transplantation, Heterologous Tumor Cells, Cultured Tumor Suppressor Proteins (genetics)

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