Normal expression and inflammation-induced changes of Na and Na/K ATPase activity in spinal dorsal horn of the rat.

Abstract

We tested whether that peripheral inflammation induces changes in the spinal dorsal horn ATPase activity. Adult Sprague-Dawley rats were anesthetized (thiobarbital), the left hind paw (inflammation group; n = 15) was immersed in water at 60 degrees C for 60s, which induced a local inflammation. A control group (n = 12) was tested with water at room temperature. After 60 min of peripheral inflammation left (LDH) or right lumbar dorsal horn (RDH) were processed for total, Na/K, Na and remanent ATPase activities (nM P(i) (mgprotein)(-1) min(-1)). In control animals isoenzymatic activities were: Na (31.2%); Na/K (20.6%) and remanent (48.2%) from total ATPase activity. No LDH-RDH asymmetry was found. The inflammation group presented an ipsilateral increase of total ATPase activity in LDH (X+/-S.E.M.; 4798.9+/-601) over the RDH (3982.2+/-451; Delta+817; P<0.05). This is due to an increase in Na ATPase activity (1609.3+/-297) over RDH (1164.2+/-166; Delta+445; P<0.05). ATPase activities were increased in LDH from inflamed over the control group as follows: total (4798.9+/-601; Delta+840; P<0.05), Na/K (1298.1+/-301; Delta+483; P<0.05) and Na (1609.3+/-297; Delta+373; P<0.05). These increased ATPase activities, induced in a short time, can be considered a functional marker of nociceptive neuronal activity.

• Authors: Mariana Czaplinski, Cilia Abad, Antonio Eblen-Zajjur
• Journal: Neuroscience letters (Neurosci Lett) Vol. 374 Issue 2 Pg. 147-51 (Feb 10 2005) ISSN: 0304-3940 [Print] Ireland
• PMID: 15644282 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cation Transport Proteins
• Adenosine Triphosphatases
• sodium-translocating ATPase
• Sodium-Potassium-Exchanging ATPase

Topics

• Adenosine Triphosphatases (metabolism)
• Animals
• Cation Transport Proteins (metabolism)
• Functional Laterality (physiology)
• Hot Temperature (adverse effects)
• Inflammation (etiology, metabolism)
• Male
• Rats
• Rats, Sprague-Dawley
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Spinal Nerve Roots (enzymology, metabolism)