Potential anti-tumor promoting activity of lupane-type triterpenoids from the stem bark of Glochidion zeylanicum and Phyllanthus flexuosus.

Abstract

Four known lupane-type triterpenoids, glochidonol (1), glochidiol (2), lup-20(29)-ene-1beta,3beta-diol (3) and glochidone (3) were isolated from the stem bark of Glochidion zeylanicum. Previously, lupeol (5), lup-20(29)-ene-3beta,24-diol (6) and betulin (7) were isolated from the stem bark of Phyllanthus flexuosus. This study reports the assays of these lupane-type triterpenoids: all isolates 1-7 and synthetic analogues, glochidonyl acetate (1a), lup-20(29)-ene-1,3-dione (1b) and lup-20(29)-ene 3beta,24-diacetate (6a) were tested for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA). Among them, the effects of compounds 2 (IC50 = 290 mol ratio/32 pmol TPA) and 3 (IC50 = 300) were stronger than the others. In addition, compound 2 exhibited a strong inhibitory effect on mouse skin tumor promotion in an in vivo mouse two-stage carcinogenesis test.

• Authors: Reiko Tanaka, Yoshitaka Kinouchi, Shun-ichi Wada, Harukuni Tokuda
• Journal: Planta medica (Planta Med) Vol. 70 Issue 12 Pg. 1234-6 (Dec 2004) ISSN: 0032-0943 [Print] Germany
• PMID: 15643564 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Triterpenes
• lupane
• 9,10-Dimethyl-1,2-benzanthracene
• Tetradecanoylphorbol Acetate

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Administration, Cutaneous
• Animals
• Antigens, Viral (drug effects)
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology, therapeutic use)
• Female
• Herpesvirus 4, Human (drug effects, immunology)
• Inhibitory Concentration 50
• Mice
• Mice, Inbred ICR
• Papilloma (chemically induced, prevention & control)
• Phyllanthus
• Phytotherapy
• Plant Bark
• Plant Extracts (administration & dosage, pharmacology, therapeutic use)
• Skin Neoplasms (chemically induced, prevention & control)
• Specific Pathogen-Free Organisms
• Tetradecanoylphorbol Acetate
• Triterpenes (administration & dosage, pharmacology, therapeutic use)