Abstract |
Skin hyperpigmentations such as melasma, freckles and senile lentigines can be subjectively treated by depigmenting agents. In our ongoing study to find melanogenesis inhibitors from natural sources, Piper longum L (fruits, Piperaceae) was discovered to have an inhibitory effect on alpha-melanocyte-stimulating hormone ( alpha-MSH)-induced melanogenesis in melanoma B16 cells. Piperlonguminine has been identified as the melanogenesis inhibitor from P. longum by activity-guided extraction and isolation. The compound showed dose-dependent inhibitory effects with 85.1 +/- 4.9% inhibition at 25 microM, 62.1 +/- 6.1% at 12.5 microM, 36.4 +/- 4.6% at 6.3 microM and 18.4 +/- 5.1% at 3.1 microM on alpha-MSH-induced melanogenesis, showing an IC50 value of 9.6 microM. As a positive control, kojic acid exhibited an IC50 value of 44.6 microM on the melanogenesis. As to the mode of action, piperlonguminine showed an inhibitory effect on alpha-MSH-induced tyrosinase synthesis, documented by Western immunoblot analysis. However, piperlonguminine did not show an inhibitory effect on tyrosinase activity or a direct depigmenting effect of melanin.
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Authors | Kyung Rak Min, Kyeong-Soo Kim, Jai Seup Ro, Seung Ho Lee, Jeong Ah Kim, Jong Keun Son, Youngsoo Kim |
Journal | Planta medica
(Planta Med)
Vol. 70
Issue 12
Pg. 1115-8
(Dec 2004)
ISSN: 0032-0943 [Print] Germany |
PMID | 15643542
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dioxolanes
- Melanins
- Plant Extracts
- alpha-MSH
- Monophenol Monooxygenase
- piperlonguminine
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Topics |
- Animals
- Cell Line, Tumor
(drug effects)
- Dioxolanes
(administration & dosage, pharmacology, therapeutic use)
- Dose-Response Relationship, Drug
- Fruit
- Melanins
(biosynthesis)
- Mice
- Monophenol Monooxygenase
(biosynthesis)
- Phytotherapy
- Pigmentation Disorders
(drug therapy)
- Piper
- Plant Extracts
(administration & dosage, pharmacology, therapeutic use)
- alpha-MSH
(antagonists & inhibitors, drug effects)
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